文献类型：期刊文献

英文题名：Programmed co-assembly of DNA-peptide hybrid microdroplets by phase separation

作者：Yao, Shengtao[1];Liao, Yue[1];Pan, Rizhao[1];Zhu, Weiping[1];Xu, Yufang[1];Yang, Yangyang[1];Qian, Xuhong[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：33

期号：3

起止页码：1545

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000772416400074)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31600802).

语种：英文

外文关键词：Self-assembly; Microdroplet; DNA origami; Phase separation; Elastin-like polypeptides

摘要：Biopolymers, including DNA and peptides have been used as excellent self-assembling building blocks for programmable single-component or hybrid materials, due to their controlled molecular interactions. However, combining two assembling principles of DNA-based programmability and peptide-based specific molecular interactions for hybrid structures to microscale has not yet been achieved. In this study, we describe a hybrid microsystem that emerges from the co-assembly of DNA origami structure and short elastin-like polypeptide conjugated oligonucleotides, and initiates liquid-liquid phase separation to generate microdroplets upon heating above the transition temperature. Moreover, the hybrid microdroplets are capable for guest molecule trapping and perform bi-/tri-enzymatic cascades with rate enhancements as open "microreactors". Our programmed assembled DNA-peptide microsystem represents a new combination of DNA nanotechnology and peptide science and opens potential application routes toward lifeinspired biomaterials. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.