文献类型：期刊文献

英文题名：Rapid prediction and optimization of concentration conditions for preparative fractions by solid-phase extraction

作者：Jin, Yu[1];Xue, Xing-ya[1];Zhang, Fei-fang[1];Zhang, Jing[1];Shi, Hui[2];Xiao, Yuan-sheng[1];Ke, Yan-xiong[2];Liang, Xin-miao[1,2]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai, Peoples R China

年份：2008

卷号：31

期号：4

起止页码：615

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20081311175081);WOS:【SCI-EXPANDED(收录号:WOS:000254137900003)】；

语种：英文

外文关键词：concentration; optimization; prediction; preparative fractions; SPE

摘要：SPE is an effective tool for concentrating preparative fractions isolated from a complex sample. To guarantee high efficiency and recovery of concentration, the concentration conditions could be optimized by predicting the breakthrough volume (V-B). In this study, a method of predicting V-B of unknown compounds in preparative fractions at any isocratic mobile phase composition with the analytical retention parameters a and c is described. The a and c values and the relationship between half peak width (W-1/2) and retention time of a model analyte were measured using the analytical elution mode on an SPE column, and the V-B and retention volume (V-R) predicted with the a and c values were validated with breakthrough experiments. However, it is impossible to measure the a and c values of multiple compounds in a complex system directly on an SPE column with a low number of theoretical plates. The correlation of the a and, c values between the SPE and analytical columns was developed so that the analytical data could be transferred to the SPE column. With the calculated a and c values, we could optimize the concentration conditions on the basis of the predicted V-B and the volume of the preparative fraction.