文献类型：期刊文献

英文题名：Optimization of nutrients for dinactin production by a marine Streptomyces sp from the high latitude Arctic

作者：Zhou, Jiao[1];He, Hao[1];Wang, Xiaolong[1];Lu, Jian[1];Zhou, Xiangshan[1];Cai, Menghao[1];Zhang, Yuanxing[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2015

卷号：20

期号：4

起止页码：725

外文期刊名：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

收录：;EI(收录号：20154001327306);WOS:【SCI-EXPANDED(收录号:WOS:000361878400013)】；

基金：This work was supported by the National High Technology Research and Development Program of China (2012AA092105, 2012AA092103). We thank Prof. Bo Chen, Polar Research Institute of China, for providing the strain and general assistance.

语种：英文

外文关键词：arctic actinomycete; fermentation; dinactin; medium optimization; precursor-feeding strategy

摘要：Streptomyces sp. R-527F, which produces the macrotetrolide antibiotic dinactin, was isolated from the sediments of the Arctic Ocean. In this work, optimization of the nutrients required for dinactin production including medium development and precursor stimulation, were investigated. Optimization of the medium and replacement of polar sea water were achieved using a one factor at a time experiment in conjunction with statistical analysis using methods covering Plackett-Burman design, the steepest descent method and central composite design. Dinactin production in the optimized medium was 160.8 mg/L, which was 47 fold higher than the control. Supplementation of the fermentation with exogenous acetate (1.5 mmol/L), succinate (6 mmol/L), malonate (24 mmol/L) and citrate (6 mmol/L) further enhanced dinactin biosynthesis by 42.7, 122.3, 66.7, and 62.1%, respectively. The precursors, in particular succinate, facilitated sugar use and also increased pH levels. Furthermore, a six-pulse feeding of total 6 mmol/L succinate in a 5 L bioreactor fermentation yielded a maximal production of 279.0 mg/L dinactin, 124.1% higher than that without precursor stimulation. This nutritional regulation process is easy to scale up and holds the potential for adaptation to industrial use.