文献类型：期刊文献

中文题名：重组肺炎克雷伯菌发酵联产3-羟基丙酸和1,3-丙二醇

英文题名：Co-production of 3-Hydroxypropionie Acid and 1,3-Propanediol by a Recombinant Strain of Klebsiella pneumoniae

作者：李清[1];黄艳娜[1];李志敏[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2014

卷号：14

期号：1

起止页码：133

中文期刊名：过程工程学报

外文期刊名：The Chinese Journal of Process Engineering

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家高技术研究发展计划(863)基金资助项目(编号:2012AA022104)

语种：中文

中文关键词：肺炎克雷伯氏菌;3;羟基丙酸;1,3;丙二醇;辅因子再生;发酵;优化

外文关键词：Klebsiella pneumoniae; 3-hydroxypropionic acid; 1,3-propanediol; co-factor regeneration; fermentation; optimization

摘要：对表达了高效醛脱氢酶的重组肺炎克雷伯氏菌以甘油为底物生产3．羟基丙酸和1，3-丙二醇的过程进行优化，将发酵过程中补料阶段甘油浓度分别控制为0-10，10-20，20-30g／L，并分3次间歇性补加甘油．结果表明，发酵过程中补料阶段控制甘油浓度在20～30g／L，发酵26h得到47．20g／L3-羟基丙酸和43．90g／L1，3一丙二醇；而间歇性补加甘油产物得率最高，发酵26h时3．羟基丙酸和1,3-丙二醇相对甘油的得率分别为0．35和0．38mol／m01．3-羟基丙酸和1，3-丙二醇联产可实现辅因子烟酰胺腺嘌呤二核苷酸的再生平衡，从而提高碳回收率．
The production of 3-hydroxypropionic acid （3-HP） and 1,3-propanediol （1,3-PDO） by recombinant Klebsiella pneumoniae over-expressed aldehyde dehydrogenase was optimized with glycerol as substrate. During the feeding stage of fermentation, glycerol concentration was controlled differently at 0-10, 10-20, 20-30 g/L and intermittent addition of glycerol for three times. The results showed that the process controlling of glycerol concentration at 20-30 g/L resulted in the highest titer, 47.20 g/L of 3-HP and 43.90 g/L of 1,3-PDO were obtained in 26 h. The intermittent addition of glycerol led to the highest yield of 3-HP and 1,3-PDO, 0.35 and 0.38 mol/mol, respectively. The co-production of 3-HP and 1,3-PDO could achieve cofactor regeneration of NAD＋ and NADH balance, which enhanced the recovery of carbon.