文献类型：期刊文献

英文题名：An in situ photocrosslinkable platelet rich plasma - Complexed hydrogel glue with growth factor controlled release ability to promote cartilage defect repair

作者：Liu, Xiaolin[1];Yang, Yunlong[1];Niu, Xin[1];Lin, Qiuning[2];Zhao, Bizeng[1];Wang, Yang[1];Zhu, Linyong[2]

机构：[1]Shanghai Jiao Tong Univ, Inst Microsurg Extrem, Inst Orthopaed Surg, Affiliated People Hosp 6, 600 Yishan Rd, Shanghai 200233, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Adv Mat, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：62

起止页码：179

外文期刊名：ACTA BIOMATERIALIA

收录：;EI(收录号：20215111340672);WOS:【SCI-EXPANDED(收录号:WOS:000413175200013)】；

基金：This work was financially supported by the National Nature Science Foundation of China (Project Nos. 81672254, 81572120, 81472152).

语种：英文

外文关键词：Platelet-rich plasma; Photocrosslinkable hydrogel; Cartilage defect repair; Regenerative medicines

摘要：The repair of articular cartilage injury is a great clinical challenge. Platelet-rich plasma (PRP) has attracted much attention for the repair of articular cartilage injury, because it contains various growth factors that are beneficial for wound repair. However, current administration methods of PRP have many shortcomings, such as unstable biological fixation and burst release of growth factors, all of which complicate its application in the repair of articular cartilage and compromise its therapeutic efficacy. In this study, based on our previously reported photoinduced imine crosslinking (PIC) reaction, we developed an in situ photocrosslinkable PRP hydrogel glue (HNPRP) through adding a photoresponsive hyaluronic acid (HA-NB) which could generate aldehyde groups upon light irradiation and subsequently react with amino groups, into autologous PRP. Our study showed that HNPRP hydrogel glue was cytocompatible and could be conveniently and rapidly prepared in situ, forming a robust hydrogel scaffold. In addition, our results demonstrated that HNPRP hydrogel not only achieved controlled release of growth factors, but also showed strong tissue adhesive ability. Therefore, HNPRP hydrogel was quite suitable for cartilage defect regeneration. Our further in vitro experiment showed that HNPRP hydrogel could promote the proliferation and migration of chondrocytes and bone marrow stem cells (BMSCs). In vivo testing using a rabbit full-thickness cartilage defect model demonstrated that HNPRP hydrogel could achieve integrative hyaline cartilage regeneration and its therapeutic efficacy was better than thrombin activated PRP gel. Statement of Significance In this study, we have developed a photocrosslinkable platelet rich plasma (PRP) - complexed hydrogel glue (HNPRP) for cartilage regeneration. The in situ formed HNPRP hydrogel glue showed not only the controlled release ability of growth factors, but also strong tissue adhesiveness, which could resolve the current problems in clinical application of PRP. Furthermore, HNPRP hydrogel glue could promote integrative hyaline cartilage regeneration, and its reparative efficacy for cartilage defect was better than thrombin activated PRP gel. This study provided not only an effective repair material for cartilage regeneration, but also developed an advanced method for PRP application. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.