文献类型：期刊文献

中文题名：转基因聚球藻7942培养中光传递及其对细胞生长的影响

英文题名：Light Transfer and Its Effect on Cell Growth in the Culture of Transgenic Synechococcus sp. PCC7942

作者：朱笃[1];李元广[1];叶勤[1];魏晓东[1];章军[2]

机构：[1]华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室,上海200237;[2]厦门大学生命科学学院,福建厦门361005

年份：2003

卷号：3

期号：3

起止页码：231

中文期刊名：过程工程学报

外文期刊名：The Chinese Journal of Process Engineering

收录：CSTPCD;;EI(收录号：2003297554151);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家海洋863基金资助项目(编号:819-Q01);上海市科技启明星计划;上海市重点学科部分资助

语种：中文

中文关键词：微藻;转基因聚球藻;光衰减;光分布;平均光强;分批培养;光生物反应器;人胸腺素α1;细胞生长

外文关键词：light attenuation; light distribution; average irradiance; batch culture; photobioreactor; human thymosin a1; transgenic Synechococcus sp. PCC7942

摘要：对转人源胸腺素α1基因聚球藻7942光生物反应器分批培养过程中光的分布、平均光强变化及其对藻细胞生长的影响进行了分析. 结果表明,Hyperbolic光衰减模型较之Lambert-Beer光衰减模型能更好地描述转基因聚球藻7942培养液中的光衰减;随着藻细胞密度的增加,光生物反应器内平均光强不断减小,藻细胞的光能比吸收速率也不断降低,反应器中'暗区'体积不断加大;培养时间0~1.5 d,藻细胞能获得充足光能,因而呈指数生长;1.5 d后藻细胞处于光限制,比生长速率不断下降,但体积光能吸收速率达到最大并保持恒定,藻细胞进入线性生长期;5 d后光生物反应器'暗区'体积超过了反应器总体积的50%,藻细胞吸收的光能用于维持的比例不断增加,从而导致生长速率下降;培养8 d,转基因聚球藻7942中人源胸腺素α1表达量为4.53 mg/L.
The variation of light distribution and average irradiance in a photobioreactor during batch culture of transgenic Synechococcus sp. PCC7942 carrying thymosin a1 gene was modeled, and their effects on algal cell growth were analyzed. Light attenuation in the suspension culture of the transgenic Synechococcus sp. PCC7942 could be predicted more accurately with a hyperbolic model than with the Lambert-Beer model. With algal cell concentration increasing, the average irradiance in the photobioreactor and the specific light energy absorbance rate decreased continuously, and illumination zone decreased gradually after 1.5 d cultivation, resulting in the reduction of the cell specific growth rate; since light energy absorbed in the volume unit was constant after 1.5 d, the algal cell was in the linear growth phase; with dark zone exceeded 60% of the volume of the photobioreactor after 5 d cultivation, energy used for cell maintenance increased and the cell growth rate decreased. The thymosin a1 expression level of recombinant microalgal cell was 4.53 mg/L for 8 d cultivation.