文献类型：期刊文献

英文题名：Separation of antibody IgG201 by an aqueous two-phase system with recyclable pH-responsive polymers

作者：Chen, Xi[1];Guo, Yibo[1];Yang, Ting[1];Wan, Junfen[1];Cao, Xuejun[1]

机构：[1]East China Univ Sci & Technol, Dept Bioengn, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：113

起止页码：125

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20220111424023);WOS:【SCI-EXPANDED(收录号:WOS:000742550900005)】；

基金：The authors would like to thank the National Natural Science Foundation of China (No. 21376078) . Technical help from the analysis center of the East China University of Science and Technology (Shanghai, China) was also highly appreciated.

语种：英文

外文关键词：Monoclonal antibody purification; Aqueous two-phase system; Recyclable pH-response polymers

摘要：Since their clinical application success, therapeutic monoclonal antibodies (mAbs) have been playing important roles in the treatment of many diseases. A significant obstacle to reducing antibody drug costs is reducing the use of expensive materials in the purification manufacturing process. New technologies, such as aqueous two-phase systems (ATPSs), are an option to solve this problem. In this study, a recyclable aqueous two-phase system was developed. Two pH-responsive polymers were synthesized and characterized. The phase-forming polymers and salt concentrations, system pH, and temperature were studied. Finally, under conditions of 6% P-MDB3.36 with 20 mmol/L sodium acetate and 30 mmol/L Na2SO4 at pH 5.5, the recovery of target monoclonal antibody IgG201 was 94.4 % and partition coefficient was 8.7. The purity was 92.2 %, as determined bysize exclusion chromatography (SEC). The pH-sensitive polymers in this ATPS can be recycled near their isoelectric point.