文献类型：期刊文献

英文题名：Rapid purification of diastereoisomers from Piper kadsura using supercritical fluid chromatography with chiral stationary phases

作者：Xin, Huaxia[1];Dai, Zhuoshun[1];Cai, Jianfeng[1];Ke, Yanxiong[1];Shi, Hui[1];Fu, Qing[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

年份：2017

卷号：1509

起止页码：141

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20172503806186);WOS:【SCI-EXPANDED(收录号:WOS:000407868800016)】；

基金：This work was supported by National Natural Science Funds (81403100) and Fundamental Research Funds for the Central Universities (222201717016)

语种：英文

外文关键词：Chiral supercritical fluid chromatography; Achiral separation; Diastereoisomers; Purification; Piper kadsura

摘要：Supercritical fluid chromatography (SFC) with chiral stationary phases (CSPs) is an advanced solution for the separation of achiral compounds in Piper kadsura. Analogues and stereoisomers are abundant in natural products, but there are obstacles in separation using conventional method. In this paper, four lignan diastereoisomers, (-)-Galbelgin, (-)-Ganschisandrin, Galgravin and (-)-Veraguensin, from Piper kadsura were separated and purified by chiral SFC. Purification strategy was designed, considering of the compound enrichment, sample purity and purification throughput. Two-step achiral purification method on chiral preparative columns with stacked automated injections was developed. Unconventional mobile phase modifier dichloromethane (DCM) was applied to improve the sample solubility. Four diastereoisomers was prepared at the respective weight of 103.1 mg, 10.0 mg, 152.3 mg and 178.6 mg from 710 mg extract with the purity of greater than 98%. (C) 2017 Elsevier B.V. All rights reserved.