文献类型：期刊文献

中文题名：蟾毒灵纳米结构脂质载体的制备及性质研究

英文题名：Preparation and Characterization of Bufalin Nanostructure Lipid Carrier

作者：田振芬[1];吕文华[1];龚亚斌[2];张照悦[3];陈蕴琪[3];邹爱华[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]上海中医药大学附属岳阳中西医结合医院,上海200437;[3]华东理工大学中德工学院,上海200237

年份：2017

卷号：80

期号：7

起止页码：679

中文期刊名：化学通报

外文期刊名：Chemistry

收录：北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(21573070)资助

语种：中文

中文关键词：蟾毒灵;纳米结构脂质载体;缓释;毒性

外文关键词：Bufalin （BUF） , Nanostructure lipid carrier （NLC） , Sustained release, Toxicity

摘要：本文采用高压均质法成功将脂溶性药物蟾毒灵(BUF)负载于纳米结构脂质载体(NLC)中,并对其物理化学性质、体外释放性质及体外肿瘤细胞毒性等进行了研究。结果表明,制备获得的蟾毒灵纳米结构脂质载体(BUF-NLC)粒径小、分散度和稳定性好,具有明显的药物缓释作用。MTT实验表明,将BUF负载于NLC后,作用初期对肿瘤细胞的毒性作用低于原药,作用时间延长,毒性与原药达到一致,说明NLC的包裹并未降低蟾毒灵的药效,而是逐步释放出来,这对克服临床应用中蟾毒灵的高毒副作用有非常重要的意义。
In this paper, Bufalin loaded nanostructure lipid carrier （BUF-NLC） was successfully prepared by high pressure homogenization. The physicochemical properties, in vitro release and cytotoxicity （MTT） were characterized. The results indicated that BUF-NLC has small particle size, good dispersibility and stability. The MTT test suggested that the cytotoxicity of BUF-NLC on tumor cells was lower than free BUF in the initial stage, but after prolonged the action time, the cytotoxicity of BUF-NLC was consistent with that of BUF, indicating that NLC package does not reduce the cytotoxicity of BUF, but gradually release it, which was very important to overcome the side- effects caused by the high toxicity of BUF.