文献类型：期刊文献

中文题名：基于负载过氧化氢酶的介孔二氧化硅粒子的酶场效应晶体管生物传感器

英文题名：Enzyme Field Effect Transistor Biosensor Based on Horseradish Peroxidase-loaded Mesoporous Silica Particles

作者：朱以华[1];王平[1];杨晓玲[1]

机构：[1]华东理工大学超细材料制备与应用教育部重点实验室

年份：2009

卷号：15

期号：1

起止页码：17

中文期刊名：中国粉体技术

外文期刊名：China Powder Science and Technology

收录：CSTPCD;;北大核心:【北大核心2008】；

基金：国家自然科学基金项目,编号:20676038;教育部科学技术研究重点项目,编号:107045

语种：中文

中文关键词：介孔二氧化硅;辣根过氧化氢酶;酶场效应管;生物传感器

外文关键词：mesoporous silica;horseradish peroxidase ; enzyme field effect transistor;biosensors

摘要：制备了基于负载辣根过氧化氢酶（HRP）的介孔二氧化硅（MS）粒子的酶场效应晶体管（ENFET）用来检测过氧化氢浓度的生物传感器。不同孔径的MS粒子被用作固定酶的载体构造ENFET，显著改善了酶的固定量。用负载HRP的MS粒子修饰于离子敏场效应管的栅极制成的过氧化氢敏ENFET灵敏度较传统的直接固定HRP的ENFET有显著的提高。对不同孔径的MS粒子研究发现，扩孔的MS粒子的性能更好，同时还讨论了缓冲液浓度和pH值的影响。另外，由于在MS粒子表面组装了5个复层的聚电解质，生物传感器的稳定性良好。
The enzyme field effect transistor （ENFET） biosensor based on horseradish peroxidase （ HRP ）loaded mesoporous silica （MS）particles for detecting H2O2 concentration was fabricated. The MS particles of different pore sizes were used as the substrates for immobilizing enzyme to construct ENFETs. The MS particles showed significantly improved enzyme immo- bilization capacity. The H2O2 sensitive ENFET based on the modification of the gate surface of ionsensitive field effect transistors （ISFETs）with HRP loaded MS particles showed obviously enhanced sensitivity compared with the conventional ones. MS particles of different pore sizes were investigated and the sensor with remodeled MS particles which experienced the pore expanding treatment showed better performance than untreated MS particles. The influences of buffer concentration and pH were discussed. The biosensor also had good stability owing to assembling 5 bilayers of polyelectrolyte shell on the MS particles＇ surfaces.