文献类型：期刊文献

英文题名：Antibacterial Peptide Secreted by Pediococcus acidilactici Enables Efficient Cellulosic Open L-Lactic Acid Fermentation

作者：Qureshi, Abdul Sattar[1,2];Zhang, Jian[1];Sousa, Leonardo da Costa[3,4];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Univ Sindh, Inst Biotechnol & Genet Engn, Jamshoro 76080, Sindh, Pakistan;[3]Michigan State Univ, Dept Chem Engn & Mat Sci, GLBRC, E Lansing, MI 48824 USA;[4]Michigan State Univ, DOE, GLBRC, E Lansing, MI 48824 USA

年份：2017

卷号：5

期号：10

起止页码：9254

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20174104242082);WOS:【SCI-EXPANDED(收录号:WOS:000412382700084)】；

基金：This research was supported by the National Key Research and Development Program of China (2017YFB0309302), the Natural Science Foundation of China (21306048), the Fundamental Research Funds for the Central Universities of China (WF1514325), the Open Funding Project of the Key Laboratory of Motor Vehicle Biofuel Technology (KFKT2014011), and the Chinese Scholarship Council (CSC NO. 201506745002). L.D.C.S. also acknowledges the DOE Great Lakes Bioenergy Research Center (http://www.greatlakesbioenergy.org) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through Cooperative Agreement DE-FC02-07ER64494 between the Board of Regents of the University of Wisconsin System and the U.S. Department of Energy.

语种：英文

外文关键词：Microbial contamination; Open fermentation; Pediococcus acidilactici TY112; High solids loading; Simultaneous saccharification and fermentation (SSF); Corn stover

摘要：Maintaining aseptic conditions is one of the major cost factors in industrial fermentation. Here, we have demonstrated an open fermentation method for producing cellulosic L-lactic acid by Pediococcus acidilactici, which is also capable of secreting an antibacterial peptide to control potential microbial contamination. The peptide secreted by P. acidilactici TY112 was isolated and its molecular weight was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). P. acidilactici TY112 was applied to an open L-lactic acid fermentation without sterilizing reactors, medium and supporting facilities. No contamination was observed and high L-lactic acid fermentation performances were obtained during open fermentation in synthetic medium and corn stover hydrolysates, both in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) modes. The open SSF at 30% (w/w) solids loading reached 97.3 g/L of L-lactic acid with a productivity of 1.5 g/(L.h) and yield of 69.4%, which were comparable to the conventional SSF with sterilization. The antibacterial peptide secreted by P. acidilactici enabled a successful and more cost-effective open L-lactic acid fermentation method, without energy-consuming sterilization protocols. Potentially, the open fermentation concept presented herein can be applied to other fermentative processes in the future, by expressing appropriate antibacterial peptides in the desired microbial strains.