文献类型：期刊文献

英文题名：Myoglobin-Catalyzed Efficient In Situ Regeneration of NAD(P)+ and Their Synthetic Biomimetic for Dehydrogenase-Mediated Oxidations

作者：Jia, Hao-Yu[1];Zong, Min-Hua[1];Zheng, Gao-Wei[2];Li, Ning[1]

机构：[1]South China Univ Technol, Sch Food Sci & Engn, 381 Wushan Rd, Guangzhou 510640, Guangdong, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2019

卷号：9

期号：3

起止页码：2196

外文期刊名：ACS CATALYSIS

收录：;EI(收录号：20191006591910);WOS:【SCI-EXPANDED(收录号:WOS:000460600600053)】；

基金：This research was financially supported by the National Natural Science Foundation of China (21676103), the Natural Science Foundation of Guangdong Province (2017A030313056), and the Science and Technology Project of Guangzhou City (201804010179).

语种：英文

外文关键词：cofactor regeneration; dehydrogenases; enzyme catalysis; enzyme catalytic promiscuity; oxidation

摘要：On the basis of its catalytic promiscuity, wild-type myoglobin (Mb) from horse heart was used for in situ regeneration of oxidized nicotinamide cofactors. Mb proved to be a versatile catalyst, since it was capable of efficient oxidation of both natural cofactors NAD(P)H and their synthetic biomimetic BNAH with H2O2. Mb-catalyzed oxidation of reduced nicotinamide cofactors was promoted significantly in the presence of mediators such as scopoletin and acetaminophen. A cofactor total turnover number (TTN) up to 50 000 was obtained in glucose dehydrogenase-catalyzed oxidation of glucose coupled with the Mb/scopoletin regeneration system. Approximately 4 mol of glucose was enzymatically oxidized with 1 mol of H2O2. Besides, this system is well compatible with various dehydrogenases. The desired products were afforded with yields of 93-97% in dehydrogenase-catalyzed oxidations. A radical mechanism was proposed for Mb/scopoletin-catalyzed recycling of NAD(P)(+). The Mb-based recycling system may create opportunities for academic and industrial biocatalytic applications.