文献类型：期刊文献

英文题名：Analysis of the Transient Current in Nanopores Using a Circle-Median Filter

作者：Shen, Bin[1,2];Hu, Zheng-Li[3];Gu, Zhen[1];Ying, Yi-Lun[3];Wang, Hui-Feng[1];Long, Yi-Tao[3]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Control & Optimizat Chem Proc, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Informatizat Off, Shanghai 200237, Peoples R China;[3]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China

年份：2022

卷号：17

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000954846600025)】；

基金：ACKNOWLEDGMENTS This study was supported by the National Natural Science Foundation of China (Nos. 21327807, 21421004) , Program of Shanghai Subject Chief Scientist (No. 15XD1501200) and the Fundamental Research Funds for the Central Universities (Nos. 222201718001, 222201717003) .

语种：英文

外文关键词：Nanopore; Data analysis; Single molecule analysis; Circle-Median filtering

摘要：To identify and analyze the transient current jump caused by the weak interaction between biomolecules in a nanopore experiment, CM Analyzer software was designed based on the Circle-Median algorithm. The algorithm effectively recognized falling events and outliers in nanopore data. To verify the accuracy of the developed software, a simulation signal with different noise (std: 0.5 similar to 2) was generated, and results showed that the software achieves data-processing requirements with a 250-kHz sampling rate and high noise. The algorithm was also used in alpha-hemolysin biological nanopore-based single DNA molecule detection experiments, and results showed that the software can accurately identify the falling current and rising transit in single nanopore blockages.