文献类型：期刊文献

英文题名：Efficient Degradation of Malathion in the Presence of Detergents Using an Engineered Organophosphorus Hydrolase Highly. Expressed by Pichia pastoris without Methanol Induction

作者：Bai, Yun-Peng[1];Luo, Xiao-Jing[1];Zhao, Yu-Lian[2];Li, Chun-Xiu[1];Xu, Dian-Sheng[2];Xu, Jian-He[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2017

卷号：65

期号：41

起止页码：9094

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20174304288054);WOS:【SCI-EXPANDED(收录号:WOS:000413503000015)】；

基金：This work was financially sponsored by the National Key Research and Development Program of China (2016YFA0204300), National Natural Science Foundation of China (Nos. 21505044, 21506055, and 21536004), and Shanghai Pujiang Program (15PJ1401200).

语种：英文

外文关键词：biodegradation; Pichia pastoris; organophosphorus hydrolase; extracellular expression; malathion

摘要：The biodegradation of pesticides by organophosphorus hydrolases (OPHs) requires an efficient enzyme production technology in industry. Herein, a Pichia pastoris strain was constructed for the extracellular expression of PoOPHM9, an engineered malathion-degrading enzyme. After optimization, the maximum titer and yield of fermentation reached 50.8 kU/L and 4.1 g(protein)/L after 3 days, with the highest space-time yield (STY) reported so far, 640 U L-1 h(-1). PoOPHM9 displayed its high activity and stability in the presence of 0.1% (w/w) plant-derived detergent. Only 0.04 mg/mL enzyme could completely remove 0.15 mM malathion in aqueous solution within 20 min. Furthermore, 12 mu mol malathion on apples and cucumbers surfaces was completely removed by 0.05 mg/mL PoOPHM9 in tap water after 35 min washing. The efficient production of the highly active PoOPHM9 has cleared a major barrier to biodegradation of pesticide residues in food industry.