文献类型：期刊文献

英文题名：The effects of Spinosad on zebrafish larvae and THP-1 cells: Associated with immune cell damage and NF-kappa B signaling pathway activation

作者：Wang, Wei-Guo[1]; Xiong, Shou-Qian[1]; Lu, Jin[3]; Zhu, Lian-Hua[1]; Zhang, Cheng[2]; Cheng, Jia-Gao[1]; Li, Zhong[1]; Xu, Wen-Ping[1]; Tao, Li-Ming[1]; Zhang, Yang[1]

机构：[1] Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [2] Department of Pathology, UT Southwestern Medical Center, Dallas, TX, 75390, United States; [3] Frog Prince [Fujian] Baby&Child Care Product Co.,Ltd, Fujian, Zhangzhou, 363000, China

年份：2023

卷号：343

外文期刊名：Chemosphere

收录：EI(收录号：20233914776024)

语种：英文

外文关键词：Antigen-antibody reactions - Cell signaling - Cytology - Macrophages - Signaling

摘要：Spinosad is a highly effective macrolide insecticide with a wide range of applications. However, few studies have been reported on the effects of Spinosad on immune cells. The immune system is an important line of defense in the human body and plays an important role in maintaining the normal functioning of the organism. Meanwhile, macrophages, neutrophils and Thymic T cells are an important component of the immune system. We studied the immunotoxicity of Spinosad using zebrafish and THP-1 cells. In vivo, Spinosad (0–20 μM) did not cause developmental toxicity in zebrafish, but induced damage to immune cells. In vitro, Spinosad (0–20 μM) inhibited THP-1 cells viability and induced mitochondrial damage and oxidative stress production. In further studies, it impaired phagocytosis of THP-1 cells and interfered with lipid metabolism. In addition, we found that Spinosad can promote the formation of the inflammatory body NLRP3 (NLR family, pyrin domain-containing 3) and activate the NF-kappa B (NF-κB) signaling pathway. These results suggest that Spinosad has a potential risk for inducing immunotoxicity. This study has drawn attention to Spinosad-induced immunotoxicity. ? 2023 Elsevier Ltd