文献类型：期刊文献

英文题名：Metabolic Engineering of Trichoderma reesei for L-Malic Acid Production

作者：Chen, Yumeng[1];Han, Ao[1];Wang, Meng[1];Wei, Dongzhi[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：71

期号：9

起止页码：4043

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20230913648406);WOS:【SCI-EXPANDED(收录号:WOS:000935907000001)】；

基金：Funding This research was supported by the National Key Research and Development Program of China (2022YFA0912300) , National Natural Science Foundation of China (32000050) , Natural Science Foundation of Shanghai (No. 22ZR1417600) , Chenguang Program of the Shanghai Education Development Foundation, and Shanghai Municipal Education Commission (Grant No. 21CGA34) .

语种：英文

外文关键词：Trichoderma reesei; cell factory; L-malic acid; C4-dicarboxylate transporter; rTCA pathway

摘要：L-Malic acid has various applications in the chemical and food industries. The filamentous fungus Trichoderma reesei is known to be an efficient enzyme producer. Here, through metabolic engineering, T. reesei was constructed for the first time as an excellent cell factory for L-malic acid production. The heterologous overexpression of genes encoding the C4-dicarboxylate transporter from Aspergillus oryzae and Schizosaccharomyces pombe initiated L-malic acid production. The overexpression of pyruvate carboxylase from A. oryzae in the reductive tricarboxylic acid pathway further increased both the titer and yield of L-malic acid, resulting in the highest titer reported in a shake-flask culture. Furthermore, the deletion of malate thiokinase blocked L-malic acid degradation. Finally, the engineered T. reesei strain produced 220.5 g/L of L-malic acid in a 5 L fed-batch culture (productivity of 1.15 g/L/h). A T. reesei cell factory was created for the efficient production of L-malic acid.