文献类型：期刊文献

中文题名：pH和温度敏感型聚合物荧光探针的合成及应用

英文题名：Synthesis and Characterization of p H and Temperature Sensitive Polymeric Fluorescent Probe

作者：陈帆[1];郭志前[1];朱为宏[1]

机构：[1]华东理工大学精细化工研究所,上海200237

年份：2018

卷号：35

期号：4

起止页码：401

中文期刊名：应用化学

外文期刊名：Chinese Journal of Applied Chemistry

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金(21622602;21325625)项目资助~~

语种：中文

中文关键词：聚合物荧光探针;pH敏感;温度敏感;多面体笼状硅氧烷

外文关键词：polymeric fluorescent probe;pH sensitivity;temperature sensitivity;polyhedral oligomeric silsesquioxane

摘要：以萘酰亚胺(NI)、N-异丙基丙烯酰胺(NIPAM)和多面体齐聚倍半硅氧烷(POSS)为原料,通过可逆加成断裂自由基聚合(RAFT)方法,设计合成了具有p H响应和温度敏感的聚合物荧光探针Poly(POSS-NINIPAM)。通过核磁共振波谱仪(NMR)、荧光发射光谱仪和质谱仪(MS)等技术手段表征了Poly(POSS-NINIPAM)的p H和温度的响应性能。结果表明,在p H值5.8~8.0范围内,探针Poly(POSS-NI-NIPAM)的荧光强度变化明显,与p H值呈现良好的线性相关(λem=520 nm,p Ka=6.51),对p H和温度的响应均呈现出良好的循环检测性能,可用于检测活细胞He La的p H值变化。探针Poly(POSS-NI-NIPAM)在检测生物样本中p H/温度变化方面具有潜在的应用前景。
A pH and thermo-responsive fluorescent polymeric sensor Poly(POSS-NI-NIPAM)was synthesized from naphthalimide(NI),N-isopropyl acrylamide(NIPAM)and polyhedral oligomeric silsesquioxane(POSS)as raw materials by the reversible addition-fragmentation chain transfer polymerization(RAFT).The pH and thermo-responsive properties of Poly(POSS-NI-NIPAM)were characterized by nuclear magnetic resonance spectroscopy(NMR),fluorescence emission spectrometer and mass spectrometry(MS).The results show that Poly(POSS-NI-NIPAM)shows obvious fluorescence changes in the range of pH 5.8~8.0 with a reversible performance.The high sensitivity for pH/temperature makes Poly(POSS-NI-NIPAM)as a promising fluorescent probe for the detection of intracellular pH/temperature in living cells.