文献类型：期刊文献

英文题名：Development of a Multilocus Sequence Typing Tool for High-Resolution Genotyping of Enterocytozoon bieneusi

作者：Feng, Yaoyu[1];Li, Na[3];Dearen, Theresa;Lobo, Maria L.[4];Matos, Olga[4];Cama, Vitaliano[5];Xiao, Lihua[2]

机构：[1]E China Univ Sci & Technol, Sch Resource & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA;[3]Tongji Univ, Sch Life Sci & Technol, Shanghai 200295, Peoples R China;[4]Univ Nova Lisboa, Inst Higiene & Med Trop, P-1349008 Lisbon, Portugal;[5]Ctr Dis Control & Prevent, Div Parasit Dis & Malaria, Atlanta, GA 30333 USA

年份：2011

卷号：77

期号：14

起止页码：4822

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000292510400014)】；

基金：This work was supported in part by the National Natural Science Foundation of China (grants 30771881, 30928019, and 81041078), Fundamental Research Funds for the Central Universities, China (grant WB0914044), the Shanghai Science and Technology Committee (grant 09540704400), and the State Key Laboratory of Veterinary Etiological Biology at the Lanzhou Veterinary Research Institute.

语种：英文

摘要：Thus far, genotyping of Enterocytozoon bieneusi has been based solely on DNA sequence analysis of the internal transcribed spacer (ITS) of the rRNA gene. Both host-adapted and zoonotic (human-pathogenic) genotypes of E. bieneusi have been identified. In this study, we searched for microsatellite and minisatellite sequences in the whole-genome sequence database of E. bieneusi isolate H348. Seven potential targets (MS1 to MS7) were identified. Testing of the seven targets by PCR using two human-pathogenic E. bieneusi genotypes (A and Peru10) led to the selection of four targets (MS1, MS3, MS4, and MS7). Further analysis of the four loci with an additional 24 specimens of both host-adapted and zoonotic E. bieneusi genotypes indicated that most host-adapted genotypes were not amplified by PCR targeting these loci. In contrast, 10 or 11 of the 13 specimens of the zoonotic genotypes were amplified by PCR at each locus. Altogether, 12, 8, 7, and 11 genotypes of were identified at MS1, MS3, MS4, and MS7, respectively. Phylogenetic analysis of the nucleotide sequences obtained produced a genetic relationship that was similar to the one at the ITS locus, with the formation of a large group of zoonotic genotypes that included most E. bieneusi genotypes in humans. Thus, a multilocus sequence typing tool was developed for high-resolution genotyping of E. bieneusi. Data obtained in the study should also have implications for understanding the taxonomy of Enterocytozoon spp., the public health significance of E. bieneusi in animals, and the sources of human E. bieneusi infections.