文献类型：期刊文献

英文题名：A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30

作者：Zhang, Lei[1];Zhang, Suping[2];Jiang, Xin[3];Wei, Wei[1];Wang, Wei[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Ctr Biomass Energy, Shanghai 200237, Peoples R China;[3]Tianjin Hualida Biotechnol Co Ltd, TEDA, Tianjin 300457, Peoples R China

年份：2016

卷号：38

期号：1

起止页码：89

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20153601251099);WOS:【SCI-EXPANDED(收录号:WOS:000367851600010)】；

基金：This research was supported by the National Natural Science Foundation of China (No. C010302-31500066), the National High Technology Research and Development Program of China (863, Program No. 2012AA101806 and 2013AA102109), and National Basic Research Program of China (973, Program No. 2012CB721103).

语种：英文

外文关键词：Alkaline cellulose; Co-expression; Filamentous fungi; Phosphinothricin; xyn2 Promoter; Trichoderma reesei

摘要：To develop a host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei (teleomorph Hypocrea jecorina) industrial strain RUT-C30. The co-expression and purification system is based on: (i) an efficient and reliable selectable marker using bar (phosphinothricin acetyltransferase gene); (ii) a compact hygromycin B resistance marker; and (iii) a versatile integration plasmid for gene expression and knockout. Fluorescent protein genes were introduced into T. reesei and the corresponding proteins were purified from fermentation broth. The host-vector system was used in a proof-of-principle approach to achieve the co-expression of an alkaline endoglucanase and an alkaline cellobiohydrolase. This protocol can be used at an industrial scale to produce large amounts of proteins in T. reesei.