文献类型：期刊文献

英文题名：The organophosphate insecticide chlorpyrifos confers its genotoxic effects by inducing DNA damage and cell apoptosis

作者：Li, Diqiu[1];Huang, Qingchun[1];Lu, Miaoqing[1];Zhang, Lei[1];Yang, Zhichuan[1];Zong, Mimi[1];Tao, Liming[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2015

卷号：135

起止页码：387

外文期刊名：CHEMOSPHERE

收录：;EI(收录号：20152300925209);WOS:【SCI-EXPANDED(收录号:WOS:000356740900050)】；

基金：This work was financially supported by National Key Technologies R&D Program (2011BAE06B04). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Chlorpyrifos; Genotoxicity; DNA damage; Cell apoptosis; H2AX foci

摘要：The organophosphate insecticide chlorpyrifos (CPF) is known to induce neurological effects, malformation and micronucleus formation, persistent developmental disorders, and maternal toxicity in rats and mice. The binding of chlorpyrifos with DNA to produce DNA adducts leads to an increasing social concern about the genotoxic risk of CPF in human, but CPF-induced cytotoxicity through DNA damage and cell apoptosis is not well understood. Here, we quantified the cytotoxicity and potential genotoxicity of CPF using the alkaline comet assay, gamma H2AX foci formation, and the DNA laddering assay in order to detect DNA damage and apoptosis in human HeLa and HEK293 cells in vitro. Drosophila S2 cells were used as a positive control. The alkaline comet assay showed that sublethal concentrations of CPF induced significant concentration-dependent increases in single-strand DNA breaks in the treated cells compared with the control. The percentage of gamma H2AX-positive HeLa cells revealed that CPF also causes DNA double-strand breaks in a time-dependent manner. Moreover, DNA fragmentation analysis demonstrated that exposure to CPF induced a significant concentration- and time-dependent increase in cell apoptosis. We conclude that CPF is a strongly genotoxic agent that induces DNA damage and cell apoptosis. (C) 2015 Elsevier Ltd. All rights reserved.