文献类型：期刊文献

英文题名：β-Cyclodextrin-Based Inclusion Complexation Bridged Biodegradable Self-Assembly Macromolecular Micelle for the Delivery of Paclitaxel

作者：Chen, Yanzuo[1,2];Huang, Yukun[1];Qin, Dongdong[1];Liu, Wenchao[1];Song, Chao[3,4,5];Lou, Kaiyan[3,4,5];Wang, Wei[3,6];Gao, Feng[1,2,3]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[5]E China Univ Sci & Technol, State Key Lab Bioengn Reactor, Shanghai 200237, Peoples R China;[6]Univ New Mexico, Dept Chem & Chem Biol, Albuquerque, NM 87131 USA

年份：2016

卷号：11

期号：3

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000371993000073)】；

基金：This work was supported by Fundamental Research Funds for the Central Universities (WY1213013 ECUST) KL study design (http://en.moe.gov.cn/), Fundamental Research Funds for the Central Universities(22A201514055 ECUST) YC data collection and analysis (http://en.moe.gov.cn/), National Natural Science Foundation of China (81503021) YC decision to publish (http://www.nsfc.gov.cn/publish/portal1/), School of Pharmacy, Fudan University & the Open Project Program of Key Lab of Smart Drug Delivery (Fudan University), Ministry of Education (SDD2014-2) YC preparation of the manuscript (http://smartdds.fudan.edu.cn/).

语种：英文

摘要：In this study, a novel adamantanamine-paclitaxel (AD-PTX) incorporated oligochitosan-carboxymethyl-beta-cyclodextrin (CSO-g-CM-beta-CD) self-assembly macromolecular (CSO-gCM-beta-CD@AD-PTX) micelle was successfully prepared in water through sonication. The formed molecules were characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance (NMR) spectroscopy, two-dimensional NMR, elemental analysis, and liquid chromatography-mass spectrometry, while the correspondent micelles were characterized by dynamic light scattering and transmission electron microscopy. We showed that the macromolecular micelle contained a spherical core-shell structure with a diameter of 197.1 +/- 3.3 nm and zeta potential of -19.1 +/- 4.3 mV. The CSO-g-CM-beta-CD@AD-PTX micelle exhibited a high drug-loading efficacy up to 31.3%, as well as a critical micelle concentration of 3.4 x 10(-7) M, which indicated good stability. Additionally, the in vitro release profile of the CSO-g-CM-beta-CD@AD-PTX micelle demonstrated a long-term release pattern, 63.1% of AD-PTX was released from the micelle during a 30-day period. Moreover, the CSO-g-CM-beta-CD@AD-PTX micelle displayed cytotoxicity at a sub-mu M scale similar to PTX in U87 MG cells, and CSO-g-CM-beta-CD exhibited a good safety profile by not manifesting significant toxicity at concentrations up to 100 mu M. These results indicated that beta-CD-based inclusion complexation resulting in biodegradable self-assembled macromolecular micelles can be utilized as nanocarrier, and may provide a promising platform for drug delivery in the future medical applications.