文献类型：期刊文献

英文题名：A novel near-infrared fluorescent probe with a large stokes shift for the detection and imaging of biothiols

作者：Wang, Kai[1,2];Leng, Taohua[3];Liu, Yajing[4];Wang, Chengyun[1,2];Shi, Ping[4];Shen, Yongjia[1,2];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Shanghai Inst Qual Inspect & Tech Res, Natl Food Qual Supervis & Inspect Ctr Shanghai, Shanghai 200233, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：248

起止页码：338

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20171603571683);WOS:【SCI-EXPANDED(收录号:WOS:000403772200044)】；

基金：This work is sponsored by Natural Science Foundation of Shanghai (16ZR1408000), and the National Natural Science Foundation of China (No. 21576087).

语种：英文

外文关键词：Isophorone-based dye; Near-infrared fluorescence; Large stokes shift; Biothiols; Cell imaging

摘要：In this work, a dicyanoisophorone-based fluorophore, which showed an attractive near-infrared fluorescence as well as a favorable large stokes shift, was used to develop a novel probe (NIR-Probe 1) for effective detection of biothiols. It displayed distinct color changes and a turn-on NIR fluorescence response with a high off/on ratio toward biothiols. We demonstrated that NIR-Probe 1 exhibited fast sensing speed, high selectivity for biothiols and possessed the function of detecting low concentration of biothiols with impressive limit of detection: 12.1 nM for Cys, 14.5 nM for Hcy and 11.9 nM for GSH. Moreover, the utility of NIR-Probe 1 as a bioanalytical molecular tool was demonstrated by fluorescence imaging of biothiols in living cells.(C) 2017 Elsevier B.V. All rights reserved.