文献类型：期刊文献

中文题名：柑橘类黄酮成分的同步HPLC检测(英文)

英文题名：Citrus flavonoids simultaneous HPLC analysis

作者：章翀玮[1];魏东芝[1];周文瑜[1];卢艳花[1]

机构：[1]生物反应器工程国家重点实验室鲁华生物技术研究所华东理工大学,上海200237

年份：2007

卷号：29

期号：1

起止页码：99

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：酸橙;黄酮苷;多甲氧基黄酮;柚皮苷;橙皮苷;新橙皮苷;橙黄酮;川陈皮素

外文关键词：Citrus aurantium L. ; flavanone glycoside; polymethoxylated flavone; naringin; hesperidin; neohesperidin; sinensetin; nobiletin

摘要：目的:建立检测柑橘类黄酮(柚皮苷、橙皮苷、新橙皮苷、橙黄酮、川陈皮素)的中药材质量控制用快速HPLC方法。方法:用仅含甲醇和水的无盐流动相梯度洗脱,Krom asil反相分析柱(100-5C18-250A,4.6 mm×250 mm,5μm),结合二级阵列管紫外扫描做峰识别。结果:对于要检测的5种柑橘类黄酮均得到满意的分辨率和线性范围,其加样回收率:95%-102%;重现性:RSD1.88%-2.93%:分析了6种酸橙类柑橘中药材黄酮含量。结论:建立了多成分柑橘类黄酮快速HPLC检测方法并可用于LC-MS。
AIM： To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids, naringin, hesperidin, neohesperidin, sinensetin and nobiletin. METHODS： Gradient elution with non-salt mobile phase （methanol and water only） HPLC method on a Kromasil eolunm （100- 5C18-250A, 4.6 mm ×250 mm, 5 μm, C18 reverse phase） with peaks identification through DAD full UV wavelength scan. UV 284 nm and 332 nm profiles were observed. RESULTS： Satisfactory resolution, linearity, 95% - 102% of recovery and 1.88 - 2.93% of repeatability were obtained for those five citrus flavonoids. Content of 6 Citrus aurantium L. based TCM ingredients were analyzed and identified. CONCLUSION： Rapid HPLC test method on citrus flavonoids was developed and can be in LC-MS identification.