文献类型：期刊文献

英文题名：Flux Balance Analysis Inspired Bioprocess Upgrading for Lycopene Production by a Metabolically Engineered Strain of Yarrowia lipolytica

作者：Nambou, Komi[1];Jian, Xingxing[1];Zhang, Xinkai[1];Wei, Liujing[1];Lou, Jiajia[1];Madzak, Catherine[2];Hua, Qiang[1,3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]INRA, Micalis UMR1319, F-78352 Jouy En Josas, France;[3]SCICBT, Shanghai 200237, Peoples R China

年份：2015

卷号：5

期号：4

起止页码：794

外文期刊名：METABOLITES

收录：WOS:【ESCI(收录号:WOS:000367990100011)】；

基金：This study was financially supported by National Basic Research Program of China (973 Program) (2012CB721101) and National Natural Science Foundation of China (21576089).

语种：英文

外文关键词：Yarrowia lipolytica; lycopene; flux balance analysis; fermentation; metabolic network model yli v1.7

摘要：Genome-scale metabolic models embody a significant advantage of systems biology since their applications as metabolic flux simulation models enable predictions for the production of industrially-interesting metabolites. The biotechnological production of lycopene from Yarrowia lipolytica is an emerging scope that has not been fully scrutinized, especially for what concerns cultivation conditions of newly generated engineered strains. In this study, by combining flux balance analysis (FBA) and Plackett-Burman design, we screened chemicals for lycopene production from a metabolically engineered strain of Y. lipolytica. Lycopene concentrations of 126 and 242 mg/L were achieved correspondingly from the FBA-independent and the FBA-assisted designed media in fed-batch cultivation mode. Transcriptional studies revealed upregulations of heterologous genes in media designed according to FBA, thus implying the efficiency of model predictions. Our study will potentially support upgraded lycopene and other terpenoids production from existing or prospect bioengineered strains of Y. lipolytica and/or closely related yeast species.