文献类型：期刊文献

英文题名：Protein recognition by a surface imprinted colloidal array

作者：Chen, Wei[1];Lei, Wen[2];Xue, Min[1];Xue, Fei[1];Meng, Zi-hui[1];Zhang, Wei-bing[2];Qu, Feng[3];Shea, Kenneth J.[4]

机构：[1]Beijing Inst Technol, Sch Chem Engn & Environm, Beijing 100081, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China;[4]Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

年份：2014

卷号：2

期号：20

起止页码：7165

外文期刊名：JOURNAL OF MATERIALS CHEMISTRY A

收录：;EI(收录号：20141817680673);WOS:【SCI-EXPANDED(收录号:WOS:000334998400006)】；

基金：This study was supported by the National Science Foundation of China (no. 20775007, 21375009), and the National Basic Research Program of China (973 program no. 2012CB910603).

语种：英文

外文关键词：Proteins - Eye protection - Silica

摘要：A label-free and easy-to-prepare biosensor for the fast and naked-eye detection of proteins was developed in this study. A closely packed crystalline array was self-assembled in ethanol-water (1 : 1, v/v) from the surface imprinted silica (SIS) colloid for hemoglobin bovine (Hb). The reflection of the molecular imprinted colloidal array (MICA) red shifted by 23 nm in response to 1.0 mg mL(-1) of Hb at pH 7.0. Compared with the non-imprinted colloidal array (NICA), MICA has a higher selectivity towards the target protein, and can recognize the target protein via an "induced-fit" mechanism.