文献类型：期刊文献

英文题名：Production of enantiomerically pure (S)-β-phenylalanine and (R)-β-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium

作者：Li, Dengchao[1]; Cheng, Shiwei[1]; Wei, Dongzhi[1]; Ren, Yuhong[1]; Zhang, Derong[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2007

卷号：29

期号：12

起止页码：1825

外文期刊名：Biotechnology Letters

收录：EI(收录号：20074510910918)

语种：英文

外文关键词：Acylation - Enantioselectivity - Enzymes - Escherichia coli - Hydrolysis

摘要：A new approach has been developed for the production of enantiomerically pure (S)-β-phenylalanine (S-BPA) and (R)-β-phenylalanine in aqueous medium based on enantioselective acylation and hydrolysis properties of penicillin G acylase from Escherichia coli. The acylation reaction was highly preferential for the acylation of (R)-BPA to form N-phenylacetyl-(R)-BPA using phenylacetamide as an acyl donor, which was separated and then hydrolyzed to (R)-BPA by the same enzyme at pH 7.5. The optimal acylation reaction was at pH 10, 25°C with a 2:1 molar ratio of phenylacetamide to BPA, 8 IU ml -1 enzyme and 150 mM BPA. These resulted in a conversion of about 50% BPA; enantiomeric excess of (S)-BPA and (R)-BPA separated were 98 and 99%, respectively. ? 2007 Springer Science+Business Media B.V.