文献类型：期刊文献

英文题名：Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae

作者：Jiang, Jiaqi[1];Huang, Bing[1];Wu, Hui[1,2,3];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：5

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20224513077580);WOS:【SCI-EXPANDED(收录号:WOS:000461106800034)】；

基金：This study was supported by the Fundamental Research Funds for the Central Universities (Grant Nos. 222201313007, 22A201514042, 22221818014), the National High Technology Research and Development Program of China (Grant Nos. 2012AA022104, 2012AA021205), Partially Supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：3-Hydroxypropionic acid; 1,3-Propanediol; Klebsiella pneumoniae; Micro-aerobic fermentation; Scale-up

摘要：Background: 3-Hydroxypropionic acid (3-HP) is one of the top value-added chemicals that can be produced from renewable resources. Crude glycerol, a cheap feedstock, is the foremost by-product generated from biodiesel industries. In this study, Klebsiella pneumonia was engineered for efficient 3-HP production from glycerol. Results: Three metabolically engineered K. pneumoniae strains were constructed and the strain JJQ02 (Delta ldhA Delta dhaT) overexpressing aldehyde dehydrogenase from E. coil was the most suitable for 3-HP production. This strain produced 61.9 g/L of 3-HP (a yield of 0.58 mol/mol) in 38 h in a 5-L fermentor by control of the aeration rate. Deletion of IdhA and dhaTeffectively eliminated lactate formation and reduced 1,3-propanediol production, but more 2,3-butanediol was accumulated due to redox balance. The fed-batch process was successfully scaled-up in a 300-L bioreactor where the 3-HP production level was 54.5 g/L (a yield of 0.43 mol/mol) in 51 h. Conclusions: Deficiency of both lactate dehydrogenase and 1,3-propanediol oxidoreductase as well as control of aeration significantly enhanced 3-HP production, and the successful scale-up of the fed-batch process in a 300-L bioreactor indicated a great potential to industrial production of 3-HP.