文献类型：期刊文献

英文题名：Effect of substrate concentration in on the synthesis of cefaclor by penicillin acylase with in situ product removal

作者：Zhang, Ye-Wang[1]; Li, Deng-Chao[1]; Song, Qing-Xun[1]; Liu, Shu-Lai[1]; Wei, Dong-Zhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：20

期号：2

起止页码：183

外文期刊名：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY

收录：;EI(收录号：20062910006697);WOS:【SCI-EXPANDED(收录号:WOS:000240417600009)】；

语种：英文

外文关键词：acyl donor feeding; 3-chloro-7-D-(2-phenylglycinamide)-3-cephem-4-carboxylic acid; enzymatic synthesis; in situ product removal; penicillin acylase; substrate concentration

摘要：Enzymatic synthesis of 3-chloro-7-D-(2-phenylglycinamide)-3-cephem-4-carboxylic acid (cefaclor) by penicillin acylase (PA) was carried out with in situ product removal (ISPR) under kinetic control. The yield of cefaclor highly depended on substrate concentrations and the ratio of nucleus to acyl donor. Substrate concentrations were optimized as 50 mmol l(-1) of 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid (7ACCA) and 100 mmol l(-1) of phenylglycine methyl ester (PGME) at the conditions: temperature 20 degrees C, pH 6.3; and enzyme load was 8 IU ml(-1). It is effective to improve the transfer of acyl donor through controlling the substrate concentration with feeding acyl donor. The conversion of nucleus and acyl donor was improved to 93 % and 62 %, respectively.