文献类型：期刊文献

英文题名：Single Gold Nanoparticles as Real-Time Optical Probes for the Detection of NADH-Dependent Intracellular Metabolic Enzymatic Pathways

作者：Zhang, Lei[1];Li, Yang[1];Li, Da-Wei[1];Jing, Chao[1];Chen, Xiaoyuan[2];Lv, Min[3];Huang, Qing[3];Long, Yi-Tao[1];Willner, Itamar[4]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Natl Inst Biomed Imaging & Bioengn, Lab Mol Imaging & Nanomed, NIH, Bethesda, MD 20892 USA;[3]Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China;[4]Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

年份：2011

卷号：50

期号：30

起止页码：6789

外文期刊名：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

收录：;EI(收录号：20112914151460);WOS:【SCI-EXPANDED(收录号:WOS:000292774200010)】；

基金：This research is supported by the Major Research Plan of National Natural Science Foundation of China (91027035), Key Program of National Natural Science of China (20933007), and Shanghai Pujiang Program Grant of China (09PJ1403300).

语种：英文

外文关键词：gold nanoparticles; metabolic pathways; NADH; plasmon resonance Rayleigh scattering

摘要：A coat of many colors: Plasmon resonance Rayleigh scattering (PRRS) spectroscopy is applied to the in-situ monitoring of the NADH-dependent growth of Au@Cu core-shell nanoparticles (NPs). The scattering signal of the AuNPs is red shifted when they are coated with Cu (see scheme). In conjunction with dark-field microscopy, a colorimetric NADH-dependent biosensor was constructed and tested to screen an anticancer drug affecting cell NADH metabolism. Copyright ? 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.