文献类型：期刊文献

中文题名：氮源和初始NaHCO_3浓度对转Tα1基因聚球藻7942生长与表达的影响

英文题名：Effects of Nitrogen Sources and Initial NaHCO_3 Concentrations on the Growth and Thymosin α1 Expression of Transgenic Synechococcus sp. PCC7942

作者：朱笃[1];李元广[1];叶勤[1];魏晓东[1];章军[2]

机构：[1]华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室,上海200237;[2]厦门大学生命科学学院,厦门361005

年份：2003

卷号：13

期号：12

起止页码：76

中文期刊名：高技术通讯

外文期刊名：Chinese High Technology Letters

收录：CSTPCD;;EI(收录号：2004078021142);Scopus;CSCD:【CSCD2011_2012】；

基金：863计划 ( 819 Q0 1);上海市科技启明星计划;上海重点学科资助项目

语种：中文

中文关键词：NaHCO3;聚球藻;氮源;胸腺素;NaNO3;转基因技术

外文关键词：Transgenic Synechococcus sp. PCC7942, Nitrogen source, NaHCO 3, Culture, Thymosin α1

摘要：研究了氮源和初始NaHCO3浓度对转基因聚球藻 794 2生长与胸腺素α1表达的影响。结果表明 :最适的氮源为NaNO3,NaNO3浓度低于 1g/L时 ,增加NaNO3浓度能提高藻细胞的生长和表达 ,但 1～ 2 .5 g/LNaNO3对转基因聚球藻 794 2生长与表达没有明显的影响。最适的初始NaHCO3浓度为 8.0 g/L。生长在 8.0 g/L初始NaHCO3浓度培养基中藻细胞密度和胸腺素α1最大表达能力分别为BG 11培养基中的 1.94倍和 2 .0 1倍。
Effects of nitrogen sources and initial NaHCO 3 concentrations on the growth and thymosin α1 expression of transgenic Synechococcus sp. PCC7942 were studied. The results showed that NaNO 3 was the optimum nitrogen. When NaNO 3 concentration was lower than 1g/L, increasing NaNO 3 concentration could stimulate alga cells growth and improve thymosin α1 expression. While the growth and the thymosin α1 expression were not affected significantly when NaNO 3 concentrations were increased from 1g/L to 2.5g/L. The optimum concentration of NaHCO 3 was 8.0g/L. The cell density and the maximum thymosin α1 expression capability of transgenic Synechococcus sp. PCC7942 growing in the medium containing 8.0g/L NaHCO 3 were 1.94 times and 2.01 times what were obtained in BG-11 medium respectively.