文献类型：期刊文献

英文题名：One-pot synthesis of platinum-copper pyramid alloy catalyst with multiple branches for the electrochemical detection of circulating tumor DNA

作者：Chen, Kaicha[1];Zhao, Hongli[1];Wang, Zhenxing[1];Gao, Qianmei[1];Zhou, Fangfang[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2022

卷号：351

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20214411091554);WOS:【SCI-EXPANDED(收录号:WOS:000817967900006)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (19ZR1412000); the Science and Technology Commission of Shanghai Municipality (STCSM, 20520712500, 20392002300); the Fundamental Research Funds for the Central Universities (JKJ01211718).

语种：英文

外文关键词：Platinum-copper pyramid alloy; Multiple branches; Circulating tumor DNA; Signal amplification; Electrochemical biosensor

摘要：Circulating tumor DNA (ctDNA) is one of the important biomarkers for early or minimally noninvasive cancer monitoring. In this work, we constructed a ctDNA electrochemical biosensor based on a platinum-copper pyramid alloy catalyst with multiple branches (PtCu). HRTEM and XRD characterizations demonstrated that the PtCu exhibited the homogeneous morphology structure of a pyramid with multiple branches and showed a state of alloy. Further, the PtCu was used as a label of DNA signal probes (SPs) to form SPs-label via Pt-S bonds. After the immobilization of capture probes (CPs) on the gold electrode surface, the target DNA (tDNA) and SPs-label were hybridized stepwise with each other, resulting in a sandwich-like structure. Due to the special morphology and bimetallic synergistic effect, the prepared PtCu displayed outstanding catalytic capacity, which can largely amplify the current signal. By recording the catalytic current response signal of PtCu toward H2O2, the fabricated biosensor exhibited a wide linear range of 10 nM to 10 aM and a low detection limit of 7.86 aM (S/N = 3) toward tDNA. The selectivity for co-existing interference was also fascinating. Besides, the biosensor provided good stability, reproducibility, and satisfying recovery. These results demonstrated the promising application of the biosensor in ctDNA detection.