文献类型：期刊文献

英文题名：Two-step preparation of ginsenoside-Re, Rb₁, Rc and Rb₂ from the root of Panax ginseng by high-performance counter-current chromatography

作者：Cheng, Yijun[1,2];Zhang, Min[2];Liang, Qionglin[1];Hu, Ping[3];Wang, Yiming[1];Jun, Frank Wu[4];Luo, Guoan[1,2]

机构：[1]Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China;[2]E China Univ Sci & Technol, Modern Engn Ctr Tradit Chinese Med, Sch Pharm, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]XiangCam TCM Res Ctr Ltd, Cambridge CB22 3AT, England

年份：2011

卷号：77

期号：3

起止页码：347

外文期刊名：SEPARATION AND PURIFICATION TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000288738500009)】；

基金：This study was funded by the National Natural Science Foundations of China (30860381 and 20805026), the National Basic Research Program of China (973 Program, 2007CB714505) and Key Technologic Program (2006BA108B04-01). Also the authors would like to thank the colleagues at Tsinghua University, China, Mr. Xiaocheng Qi, Miss Li Chen, Dr. Qingfei Liu and Miss Haiyin Hua for their kind help and support during the research.

语种：英文

外文关键词：Counter-current chromatography; Panax ginseng; Ginsenosides; Preparation

摘要：A two-step counter-current chromatography (CCC) method was developed for the preparation of ginsenoside-Re, Rb-1, Rc and Rb-2 from ginseng with two solvent systems, methylene chloride-methanol-water-isopropanol (6:2:4:3, v/v) and n-hexane-n-butanol-0.1% formic acid (0.7:3:4, v/v). The classical CCC mode was applied in the first separation step while the elution-extrusion counter-current chromatography (EECCC) in the second separation step. The preparation yielded 76.1 mg, 137.0 mg, 58.8 mg and 85.1 mg of ginsenoside-Re, Rb-1, Rc and Rb-2 with the purity of 97.5%, 94.4%, 91.6% and 92.2%, respectively, from 1440.0 mg of crude sample. The recoveries of these ginsenosides after the two-step separation were 68.7%, 65.6%, 40.1% and 46.6%, respectively. The process throughput of crude sample was 5.14 mg/min while the process efficiencies of ginsenoside-Re, Rb-1, Rc and Rb-2 were 0.54 mg/mL, 0.49 mg/min, 0.21 mg/min and 0.30 mg/min, respectively. The structures of the targets were characterized by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) and confirmed with the standards. (C) 2011 Elsevier B.V. All rights reserved.