文献类型：期刊文献

英文题名：Isolation and Identification of a Novel Chitinilyticum sp.C8: Unleashing the Potential for Chitin Oligosaccharides Production

作者：Zhao, Qiong[1,2];Fan, Liqiang[1,2];Cui, Yanan[1,2];Zhao, Mengxuan[1,2];Deng, Chen[1,2];Zhao, Liming[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai 200237, Peoples R China

年份：2025

卷号：73

期号：19

起止页码：11829

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20251918379534);WOS:【SCI-EXPANDED(收录号:WOS:001482472000001)】；

基金：This work was supported by the Young Scientists Fund of the National Natural Science Foundation of China (32301214), the Chenguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission (23CGA39), and the National Key Research and Development Program of China (2022YFC2104500).

语种：英文

外文关键词：chitinase; chitin oligosaccharides; bacterialfermentation; biodegradation; Chitinilyticum sp.C8

摘要：Chitin oligosaccharides (CHOS) possess potential applications in food, medicine, and agriculture. Nevertheless, their low production efficiency and polymerization degree severely restrict their extensive utilization. In the present study, a Chitinase-producing bacterium was isolated and identified as Chitinilyticum sp.C8. Under the optimized conditions, the Chitinase yield reached 2.24 U/mL. Using extracellular Chitinase derived from Chitinilyticum sp.C8, chitin was converted to CHOS (DP 2-7) and GlcNAc with a yield of 96.7%. Furthermore, the endochitinase ChiC8-2 was isolated from crude enzyme preparation, which can hydrolyze chitin to obtain CHOS (DP 2-6) ((GlcNAc)2 0.2 mg/mL (22.7%), (GlcNAc)3 0.03 mg/mL (3.4%), (GlcNAc)4 0.25 mg/mL (28.4%), (GlcNAc)5 0.09 mg/mL (10.2%), and (GlcNAc)6 0.21 mg/mL (23.9%)) within 30 min. The Chitinilyticum sp.C8 could not only produce various types of chitinases but also pretreat chitin to improve the efficiency of enzymatic hydrolysis. An integrated preparation method from Chitinase production to chitin treatment by Chitinilyticum sp.C8 was developed for the efficient production of CHOS. This study introduces an environmentally friendly strategy for the production of bioactive CHOS, facilitating the sustainable utilization of renewable natural resources.