文献类型：期刊文献

英文题名：Determination of Homovanilic Acid in Human Urine by Weak Anion-Exchange Hypercrosslinked Polymer Resin-Solid Phase Extraction-High Performance Liquid Chromatography-Ultraviolet Detection Method

作者：Hu Ping[1,2];Zhang Hong-Yang[1,2];Mao Dong-Yan[1,2];Ning Fang-Hong[3];Wang Rui-Juan[2];Wang Yue-Rong[1,2];Zhang Min[4]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Biol Engn, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2012

卷号：40

期号：8

起止页码：1175

外文期刊名：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000308253700003)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 2106023), and the Shanghai Science and Technology Commission Project, China (No. 10dz222050).

语种：英文

外文关键词：Solid phase extraction; Weak anion-exchange hypercrosslinked polymer resin; High performance liquid chromatography; Urine; Homovanilic acid

摘要：A solid phase extraction-high performance liquid chromatography- ultraviolet detection (SPE-HPLC-UV) method was developed for the determination of homovanilic acid (HVA) in human urine. First, a weak anion-exchange hypercrosslinked polymer resin (HXLPP-WAX), whose adsorption performance was investigated, was synthesized as SPE packing material. And then the urinary HVA was selectively absorbed on this sorbent. After elution, the HVA was analyzed on a C-18 column with the mobile phase of methanol and aqueous acetic acid (15:85, V/V) at detection wavelength of 280 nm. The method validation results demonstrate that this method has a good linearity in the HVA concentration ranging from 2.26 to 289 mg L-1 and a limit of detection of 0.45 mg L-1. The average recoveries were above 90% and the RSD values were lower than 4.2%. The method was successfully applied to determine the HVA contents ranging from 1.55 to 6.79 mg L-1 in eight healthy human urine samples. The results showed that the proposed method was highly selective, sensitive, accurate, and reliable.