文献类型：期刊文献

英文题名：Identification of a novel transcriptional regulator, CorR, for copper stress response in Edwardsiella piscicida

作者：Xia, Feng[1];Xu, Pengfei[1];Zhang, Boya[1];Zhang, Yibei[1,2,3];Liu, Xiaohong[1,2,3];Ma, Yue[1,2,3];Zhang, Yuanxing[2,3];Wang, Qiyao[1,2,3];Shao, Shuai[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[3]Lab Aquat Anim Dis MOA, Shanghai, Peoples R China

年份：2023

卷号：89

期号：10

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;EI(收录号：20234615069376);WOS:【SCI-EXPANDED(收录号:WOS:001071847600001)】；

基金：This work was supported by grants from the National Key Research and Development Program (2022YFE0101200), the National Natural Science Foundation of China (32002436), and the China Agriculture Research System of MOF and MARA (CARS-47).r The authors declare that they have no conflicts of interest.

语种：英文

外文关键词：Edwardsiella piscicida; transposon insertion sequencing; CorR; copper; in vivo colonization

摘要：Copper plays a vital role in the host-pathogen interface, potentially making components of the bacterial copper response suitable targets for the development of innovative antimicrobial strategies. The anti-copper arsenal of intracellular pathogens has expanded as an adaptation to survive copper toxicity in order to escape intracellular killing by the host immune system. Herein, we employed transposon insertion sequencing to investigate the genetic mechanisms underlying the survival of Edward-siella piscicida under copper stress. A novel transcriptional regulator, ETAE_2324 (named CorR), was identified to participate in the response to copper ions by controlling the expression of copA, the core component of cytoplasmic copper homeostasis. Further-more, CorR regulated the expression of virulent determinant eseB, influencing the in vivo colonization of E. piscicida. Collectively, our results contribute to the comprehension of the underlying mechanism of the adaption of intracellular pathogens to copper stress during bacterial infections. IMPORTANCE Copper ions play a pivotal role in the interaction between bacteria and the host during infection. The host's innate immune system employs copper ions for their bactericidal properties, thereby making bacterial copper tolerance a crucial determinant of virulence. Edwardsiella piscicida, a significant marine pathogen, has caused substantial losses in the global aquaculture industry. To comprehensively investigate how E. piscicida responds to copper stress, we utilized transposon insertion sequencing to explore genes associated with copper tolerance in culture media containing different concentrations of copper ions. A novel transcriptional regulator, CorR, was identified to respond to copper ions and regulates the expression of crucial components of copper homeostasis CopA, along with the essential virulence factor EseB. These findings offer valuable insights into the underlying mechanisms that govern bacterial copper tolerance and present novel perspectives for the development of vaccines and therapeutic strategies targeting E. piscicida.