文献类型：期刊文献

中文题名：New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020

作者：Jiaming Yu[1];Yue Zhang[1];Li Zhang[2];Jie Shi[1];Kun Wang[1];Weize Yuan[1];Zexu Lin[1];Shangqian Ning[1];Bohao Wang[1];Xinye Wang[1];Yuyang Qiu[3];Tom Hsiang[4];Lixin Zhang[1];Xueting Liu[1];Guoliang Zhu[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;[2]Department of Chemistry,Boston University,Boston,MA,USA;[3]School of Insurance,Shandong University of Finance and Economics,Jinan,250014,China;[4]School of Environmental Sciences,University of Guelph,Guelph,Ontario,N1G 2W1,Canada

年份：2024

卷号：9

期号：4

起止页码：684

中文期刊名：Synthetic and Systems Biotechnology

外文期刊名：合成和系统生物技术(英文)

收录：CSCD:【CSCD2023_2024】；

基金：support from the National Key Research and Development Program of China(2019YFA0906200);the National Natural Science Foundation of China(21977029,81903529,21877038,31720103901,and 81573341);the Open Project Funding of the State Key Laboratory of Bioreactor Engineering,the 111 Project(B18022).

语种：英文

中文关键词：Endophytic fungi;N-acylated aminoalkanoic acid;FBMN;Adenylate-forming enzyme

摘要：Four new N-acylated aminoalkanoic acids,namely clonoroseins E?H(1?4),together with three previously identified analogs,clonoroseins A,B,and D(5?7),were identified from the endophytic fungus Clonostachys rosea strain 15020(CR15020),using Feature-based Molecular Networking(FBMN).The elucidation of their chemical structures,including their absolute configurations,was achieved through spectroscopic analysis combined with quantum chemical calculations.Bioinformatics analyses suggested that an iterative type I HR-PKS(CrsE)generates the polyketide side chain of these clonoroseins.Furthermore,a downstream adenylate-forming enzyme of the PKS(CrsD)was suspected to function as an amide synthetase.CrsD potentially facilitates the transformation of the polyketide moiety into an acyl-AMP intermediate,followed by nucleophilic substitution with eitherβ-alanine orγ-aminobutyric acid to produce amide derivatives.These findings significantly expand our understanding of PKS-related products originating from C.rosea and also underscore the powerful application of FBMN analytical methods in characterization of new compounds.