文献类型：期刊文献

英文题名：Disposable biosensor based on graphene oxide conjugated with tyrosinase assembled gold nanoparticles

作者：Song, Wei[1,2,3];Li, Da-Wei[1,2];Li, Yuan-Ting[1,2];Li, Yang[1,2];Long, Yi-Tao[1,2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China;[3]Nanjing Coll Chem & Technol, Dept Appl Chem, Nanjing 210048, Peoples R China

年份：2011

卷号：26

期号：7

起止页码：3181

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20110813685644);WOS:【SCI-EXPANDED(收录号:WOS:000288730700014)】；

基金：This work was supported by the National High Technology Research and Development Program of China (No. 2008AA06A406), the Fundamental Research Funds for the Central Universities (WK1013002), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Ministry of Health (Grant 2009 ZX 10004-301), the Shanghai Post-doctoral Sustentation Fund of China (No. 09R21411700) and the Scientific Research Project of NCCT (No. NHKY-2010-05).

语种：英文

外文关键词：Graphene oxide; Gold nanoparticles; Tyrosinase; Screen printed electrode; Disposable biosensor

摘要：A highly efficient enzyme-based screen printed electrode (SPE) was obtained by using covalent attachment between 1-pyrenebutanoic acid, succinimidyl ester (PASE) adsorbing on the graphene oxide (GO) sheets and amines of tyrosinase-protected gold nanoparticles (Tyr-Au). Herein, the bi-functional molecule PASE was assembled onto GO sheets. Subsequently, the Tyr-Au was immobilized on the PASE-GO sheets forming a biocompatible nanocomposite, which was further coated onto the working electrode surface of the SPE. The characterization of obtained nanocomposite and modified SPE surface was investigated by atomic force microscopy (AFM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Attributing to the synergistic effect of GO-Au integration and the good biocompatibility of the hybrid-material, the fabricated disposable biosensor (Tyr-Au/PASE-GO/SPE) exhibited a rapid amperometric response (less than 6 s) with a high sensitivity and good storage stability for monitoring catechol. This method shows a good linearity in the range from 8.3 x 10(-8) to 2.3 x 10(-5) M for catechol with a squared correlation coefficient of 0.9980, a quantitation limit of 8.2 x 10(-8) M (S/N= 10) and a detection limit of 2.4x 10(-8) M (S/N=3). The Michaelis-Menten constant was measured to be 0.027 mM. This disposable tyrosinase biosensor could offer a great potential for rapid, cost-effective and on-field analysis of phenolic compounds. (C) 2010 Elsevier B.V. All rights reserved.