文献类型：期刊文献

英文题名：Cytotoxic selectivity and apoptosis induction of piericidin A contributes potentially to its insecticidal effect against Mythimna separata (Lepidoptera: Noctuidae) larvae

作者：Muhayimana, Solange[1];Zhang, Xianfei[1];Xu, Jiuyong[1];Xiong, Hui[1];Luan, Shaorong[2];Zhu, Qiqi[1];Huang, Qingchun[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2019

卷号：157

起止页码：19

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000471209000003)】；

基金：This work was financially supported by the key scientific and technological project supported by Shanghai Municipal Commission of Science and Technology, China (No. 15431906800).

语种：英文

外文关键词：Piericidin A; Insecticidal activity; Cyroxic selectivity; Apoptosis; Tn5B1-4 cells

摘要：Piericidin A (PIA), an active inhibitor of Complex I, is widely used in studies of the anti-bacterial and anti-disease competence, but its physiological and mechanistic effects have rarely been clearly defined in insect individual or insect cells. The present study reveals the considerable insecticidal activity of PIA on Mythimna separata larvae by using a comparison with Aphis craccivora adult, and the cytotoxic selectivity induced by PIA on lepidopteran Tn5B1-4 cells. We demonstrate that the viability of Tn5B1-4 cells is inhibited by PIA in a time- and concentration-dependent manner with IC50 value of 0.061 mu M, whilst PIA shows slight inhibitory effect on the viability of HepG2 and Hek293 cells with IC50 value of 233.97 and 228.96 mu M, respectively. The inhibitory effect of PIA on the proliferation of Tn5B1-4 cells is significant and persistent, causing a series of morphological changes including cell shrinkage, condensed and fragmented nuclei. Intracellular biochemical assays show that PIA induces apoptosis of Tn5B1-4 cells coincides with a decrease in the mitochondrial membrane potential. PIA in Tn5B1-4 cells can be chelated by EDTA, thereby losing cytotoxicity, whereas exogenous Ca2+ restores the cytotoxicity of PIA by chelating with EDTA in a competitive manner. Our findings highlight the importance of the long-lasting cytotoxicity and the cytoxic selectivity on Tn5B1-4 cells caused by PIA, which ensure the identification of insecticidal effect of PIA against insect pests.