文献类型：期刊文献

英文题名：Simple and Sensitive Detection of HBsAg by Using a Quantum Dots Nanobeads Based Dot-Blot Immunoassay

作者：Zhang, Pengfei[1];Lu, Huiqi[1];Chen, Jia[1];Han, Huanxing[1];Ma, Wei[2,3]

机构：[1]Second Mil Med Univ, Changzheng Hosp, Translat Med Ctr, Shanghai 200433, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：4

期号：3

起止页码：307

外文期刊名：THERANOSTICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000334663900007)】；

基金：This study was supported by funds from Shanghai STCSM Project (no. 09441900700, 10dz1910302 and 11DZ1921805), and International Science & Technology Cooperation Program of China (no. S2014ZR0042). P.-F. Zhang would like to acknowledge the support from Shanghai Upper Bio-Tech Pharma Co. Ltd and the helpful discussion with Yitao Long in ECUST.

语种：英文

外文关键词：dot-blot immunoassay; HBsAg; quantum dots; nanobeads

摘要：Simple and sensitive detection of infectious disease at an affordable cost is urgently needed in developing nations. In this regard, the dot blot immunoassay has been used as a common protein detection method for detection of disease markers. However, the traditional signal reporting systems, such as those using enzymes or gold nanoparticles lack sensitivity and thus restrict the application of these methods for disease detection. In this study, we report a simple and sensitive detection method for the detection of infectious disease markers that couples the dot-blot immunoassay with quantum dots nanobeads (QDNBs) as a reporter. First, the QDNBs were prepared by an oil-in-water emulsion-evaporation technique. Because of the encapsulation of several QDs in one particle, the fluorescent signal of reporter can be amplified with QDNBs in a one-step test and be read using a UV lamp obviating the need for complicated instruments. Detection of disease-associated markers in complex mixture is possible, which demonstrates the potential of developing QDNBs into a sensitive diagnostic kit.