文献类型：期刊文献

英文题名：Fluorescent detection of copper ions with acylhydrazine-based probes: Effects of substitute and its position

作者：Wang, Hao[1];Cui, Jingjing[1];Fang, Xinhang[1];Zhang, Weibing[1];Wang, Junjun[2];Chen, Shiyang[1];Qian, Junhong[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

年份：2022

卷号：197

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20214711217641);WOS:【SCI-EXPANDED(收录号:WOS:000744244000001)】；

基金：This work was financially supported by NSFC (21576085 and 21974045) and the Open Research Fund Program of State Key Labora-tory of Biocatalysis and Enzyme Engineering (SKLBEE2020003) .

语种：英文

外文关键词：Copper ions; Thiol; Fluorescence detection; Sensing mechanism; Bioimaging

摘要：Four naphthalimide-based compounds (NHA, NHB, NHC and NHD) bearing carbazide or semi-carbazide at different positions were designed and synthesized for the detection of cooper ions. The presence of copper ions caused different spectral changes of the four compounds: the fluorescence of NHA and NHB were enhanced, while that of NHC was quenched, and little spectral changes of NHD were observed. The mechanism studies reveal that: copper ions promoted the hydrolysis of the probe containing semi-carbazide group (NHA), leading to evident fluorescence enhancement with clear red-shift; while it formed complex with the probes bearing carbazide (NHB and NHC), resulting in fluorescence "turn-on" or "turn-off". The substituted position of carbazide group in naphthalimide affected the fluorescence significantly: strong photoinduced electron transfer occurred as it at 4-position (NHB), whereas the PET process was not easy to happen as the group at imine-N (NHC). Copper ions increased the fluorescence of NHB by blocking the PET process, while it could initiate a d-PET process and quenched the fluorescence of NHC. Fluorescence bio-imaging experiments demonstrate that both NHB and NHC exhibit good cell permeability and can monitor Cu2+ as well as thiols in living cells.