文献类型：期刊文献

英文题名：Cloning and characterization of the ddsA gene encoding decaprenyl diphosphate synthase from Rhodobacter capsulatus B10

作者：Liu, Xinyi[1]; Wu, Haizhen[1]; Ye, Jiang[1]; Yuan, Qinsheng[1]; Zhang, Huizhan[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2006

卷号：52

期号：12

起止页码：1141

外文期刊名：Canadian Journal of Microbiology

收录：EI(收录号：20071810577575)

语种：英文

外文关键词：Amino acids - Bacteria - Cloning - Enzymes - Gene expression - Polypeptides

摘要：A decaprenyl diphosphate synthase gene (ddsA, GenBank accession No. DQ191802) was cloned from Rhodobacter capsulatus B10 by constructing and screening the genome library. An open reading frame of 1002 bp was revealed from sequence analysis. The deduced polypeptide consisted of 333 amino acids residues with an molecular mass of about 37 kDa. The DdsA protein contained the conserved amino acid sequence (DDXXD) of E-type polyprenyl diphosphate synthase and showed high similarity to others. In contrast, DdsA showed only 39% identity to a solanesyl diphosphate synthase cloned from R. capsulatus SB 1003. DdsA was expressed successfully in Escherichia coli. Assaying the enzyme in vivo found it made E.coli synthesize UQ-10 in addition to the endogenous production UQ-8. ? 2006 NRC.