文献类型：期刊文献

英文题名：Preparation of extracellular matrix of fish swim bladders by decellularization with supercritical carbon dioxide

作者：Han, Yuqing[1];Zhang, Bingyan[1];Li, Jinjin[1];Cen, Lian[2];Zhao, Ling[1,2];Xi, Zhenhao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem Engn, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Multiphase Mat Chem Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：10

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20230913640810);WOS:【SCI-EXPANDED(收录号:WOS:000936972100001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Grant No. 21978089), the Fundamental Research Funds for the Central Universities (Grant No. 22221818010), the Program of Shanghai Academic/Technology Research Leader (Grant No. 21XD1433000).

语种：英文

外文关键词：Supercritical carbon dioxide; Fish swim bladder; Sodium dodecyl sulfate; Decellularization; Interaction energy

摘要：Fish swim bladders used to be considered as byproducts or waste in fishery; however, they are potential materials for biological medicine with abundant collagen. In this work, an efficient noncytotoxic decellularization process using sodium dodecyl sulfate (SDS) ternary system assisted with supercritical carbon dioxide (scCO(2)) as the green extraction fluid and ethanol (ET) as the cosolvent has been developed to harvest acellular fish swim bladders (AFSBs). The experimental results show that the tissue treated by SDS assisted with scCO(2) and ethanol at 37 & DEG;C and 25 MPa can be decellularized thoroughly and maintains intact fibers and uniform pore distribution, which resulting in a tensile strength of 5.61 MPa and satisfactory biocompatibility. Meanwhile, the residual SDS content in scCO(2)/SDS/ET ternary system is 0.0122% which is significantly lower than it in scCO(2)/SDS system due to the enhanced mass transfer rate of SDS in tissues by scCO(2) with ethanol. The synergy between SDS and ethanol can enhance the diffusion coefficient and the solubility of SDS in scCO(2), which reduced the contact time between SDS and tissues. Meaningfully, the results obtained in this work can not only provide a novel strategy to produce acellular matrix with superior properties, but also offer a further understanding of the decellularization through scCO(2) extraction processing with the synergy of suitable detergent/cosolvent.