文献类型：期刊文献

英文题名：Acetylation of NarL K188 and K192 is involved in regulating Escherichia coli anaerobic nitrate respiration

作者：Cai, Shu-Shan[1];Zhang, Liu-Qing[1];Zhang, Qian[1];Ye, Bang-Ce[1];Zhou, Ying[1]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Meilong RD 130, Shanghai 200237, Peoples R China

年份：2022

卷号：106

期号：21

起止页码：7209

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20224012842740);WOS:【SCI-EXPANDED(收录号:WOS:000862213300003)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2021YFC2101500).

语种：英文

外文关键词：Acetylation; NarL; Two-component system; Anaerobic respiration; Regulation; Nitrate

摘要：As a facultative anaerobe, Escherichia coli can activate various respiratory chains during anaerobic growth, among which the mode of anaerobic respiration with nitrate allows good energy conservation. NarL is one of the regulatory proteins in the Nar two-component system that regulates anaerobic respiration in E. coli. Previous studies have shown that NarL activates downstream gene regulation through phosphorylation. However, there are few studies on other protein translational modifications that influence the regulatory function of NarL. Herein, we demonstrate that acetylation modification exists on K188 and K192, the two lysine residues involved in contacting to DNA, and the degree of acetylation has significant effects on DNA-binding abilities, thus affecting the anaerobic growth of E. coli. In addition, NarL is mainly regulated by acetyl phosphate, but not by peptidyl-lysine N-acetyltransferase. These results indicate that non-enzymatic acetylation of NarL by AcP is one of the important mechanisms for the nitrate anaerobic respiratory pathway in response to environmental changes, which extends the idea of the mechanism underlying the response of intestinal flora to changes in the intestinal environment.