文献类型：期刊文献

英文题名：Lnc-ATB contributes to gastric cancer growth through a MiR-141-3p/TGFβ2 feedback loop

作者：Lei, Kecheng[1,2];Liang, Xin[1,2];Gao, Yuwei[1,2];Xu, Baixue[1,2];Xu, Yichun[1,2];Li, Yueqi[1,2];Tao, Yiwen[3];Shi, Weibin[4];Liu, Jianwen[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Pharm, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design, Sch Pharm, Shanghai, Peoples R China;[3]Guangzhou Med Univ, Sch Pharmaceut Sci, Guangzhou, Guangdong, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Gen Surg, Shanghai, Peoples R China

年份：2017

卷号：484

期号：3

起止页码：514

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000395963900008)】；

基金：This work was supported by Science and Technology Project of Guangdong Province (2013B021100021), and Natural Science Program of Guangdong Province (2016A030313588).

语种：英文

外文关键词：Gastric cancer; lnc-ATB; miR-141-3p; TGF-beta

摘要：The long noncoding RNA (IncRNA) ATB is an important regulator in human tumors. Here, we aimed to investigate the potential molecular mechanisms of Inc-ATB in gastric cancer (GC) tumorigenesis. RT-qPCR analysis was used to detect Inc-ATB expression level in 20 pairs of gastric cancer tissues and adjacent normal gastric mucosa tissues (ANTs). Moreover, the biological role of lnc-ATB was determined in vitro. We found that lnc-ATB was significantly upregulated in GC tissues compared to Inc-ATB expression in ANTs. These high lnc-ATB expression levels predicted poor prognosis in GC patients. Low levels of Inc-ATB inhibited GC cell proliferation and cell cycle arrest in vitro. Lnc-ATB was found to directly bind miR-141-3p. Moreover, TGF-beta actives Inc-ATB and TGF-beta 2 directly binds mir-141-3p. Finally, we demonstrated that Inc-ATB fulfilled its oncogenic roles in a ceRNA-mediated manner. Our study suggests that lnc-ATB promotes tumor progression by interacting with miR-141-3p and that Lnc-ATB may be a valuable prognostic predictor for GC. In conclusion, the positive feedback loop of lnc-ATB/miR-141-3p/TGF-132 may be a potential therapeutic target for the treatment of GC. (C) 2017 Elsevier Inc. All rights reserved.