文献类型：期刊文献

中文题名：卡拉胶电荷量对牛血清蛋白/卡拉胶复合物包载姜黄素的增强作用的影响

英文题名：Influence of Carrageenan Charge on the Encapsulation Properties of Curcumin in BSA/Carrageenan Complexes

作者：徐乐颜[1,2];王凝月[2];杨明玲[3];王小永[3]

机构：[1]上海民办张江集团学校,上海201203;[2]上海市徐汇区园南小学,上海200231;[3]华东理工大学化学与分子工程学院,上海200237

年份：2019

卷号：82

期号：12

起止页码：1104

中文期刊名：化学通报

外文期刊名：Chemistry

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金项目(21573071)资助

语种：中文

中文关键词：姜黄素;蛋白质;多糖;静电吸引作用;疏水作用

外文关键词：Curcumin;Protein;Polysaccharide;Electrostatic attraction;Hydrophobic interaction

摘要：本文研究了带有不同负电荷量的κ-卡拉胶和λ-卡拉胶对牛血清蛋白(BSA)/卡拉胶复合物包载姜黄素的增强作用。卡拉胶通过与BSA之间的静电吸引作用,可以生成高稳定性的BSA/卡拉胶复合物。相比纯BSA,BSA/卡拉胶复合物能够显著提高姜黄素的溶解度和稳定性。紫外和荧光光谱表明姜黄素主要是通过疏水作用缔合于BSA的疏水微区。卡拉胶与BSA的静电吸引作用能够稳定BSA的折叠结构,从而使BSA为姜黄素提供更加疏水的缔合微环境。相比纯BSA和BSA/κ-卡拉胶复合物,带有更高负电荷的λ-卡拉胶与BSA生成的BSA/λ-卡拉胶复合物与姜黄素有更大的缔合常数和pKa1。
The influence of the different negative charges of κ-carrageenan and λ-carrageenan on the encapsulation properties of curcumin in BSA/carrageenan complexes was studied. BSA/carrageenan complexes with high stability were formed through the electrostatic attraction between BSA and carrageenan. Compared to pure BSA,BSA/carrageenan complexes could significantly increase the solubility and stability of curcumin. Absorption and fluorescence measurements indicated that curcumin is bound in the hydrophobic cavities of BSA through the hydrophobic force. The presence of carrageenan can stabilize the folded structure of BSA for providing curcumin with a more hydrophobic microenvironment. Compared to pure BSA and BSA/κ-carrageenan complexes,the values of binding constant and p Ka1 are bigger for curcumin with BSA/λ-carrageenan complexes,where λ-carrageenan carries higher negative charge than κ-carrageenan.