文献类型：期刊文献

英文题名：Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing

作者：Wang, Shuliu[1];Zeng, Xiaoqian[1];Jiang, Yue[1];Wang, Weishan[2,3];Bai, Linquan[4];Lu, Yinhua[5];Zhang, Lixin[1];Tan, Gao-Yi[1]

机构：[1]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn SKLBE, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China;[3]Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China;[5]Shanghai Normal Univ, Coll Life Sci, Shanghai 200234, Peoples R China

年份：2024

卷号：41

期号：9

起止页码：1441

外文期刊名：NATURAL PRODUCT REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001250166000001)】；

基金：This work was supported by National Natural Science Foundation of China [32370064, 31830104, 31870040 and 32121005]; the National Key Research and Development Program of China [2020YFA0907800 and 2021YFC2100600]; the 111 Project [B18022]; the Fundamental Research Funds for the Central Universities.

语种：英文

摘要：Covering: up to the end of 2023Type I CRISPR-Cas systems are widely distributed, found in over 40% of bacteria and 80% of archaea. Among genome-sequenced actinomycetes (particularly Streptomyces spp.), 45.54% possess type I CRISPR-Cas systems. In comparison to widely used CRISPR systems like Cas9 or Cas12a, these endogenous CRISPR-Cas systems have significant advantages, including better compatibility, wide distribution, and ease of operation (since no exogenous Cas gene delivery is needed). Furthermore, type I CRISPR-Cas systems can simultaneously edit and regulate genes by adjusting the crRNA spacer length. Meanwhile, most actinomycetes are recalcitrant to genetic manipulation, hindering the discovery and engineering of natural products (NPs). The endogenous type I CRISPR-Cas systems in actinomycetes may offer a promising alternative to overcome these barriers. This review summarizes the challenges and recent advances in CRISPR-based genome engineering technologies for actinomycetes. It also presents and discusses how to establish and develop genome editing tools based on type I CRISPR-Cas systems in actinomycetes, with the aim of their future application in gene editing and the discovery of NPs in actinomycetes. This review introduces the potential of type I CRISPR-Cas systems in actinomycetes for genome editing and discusses how to establish and develop genome editing tools based on type I CRISPR-Cas systems in actinomycetes.