文献类型：期刊文献

英文题名：Structural Characterization of Lipopeptides from Brevibacillus brevis HOB1

作者：Wang, Ji[1,2];Haddad, Namir I. A.[1,2];Yang, Shi-Zhong[1,2];Mu, Bo-Zhong[1,2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

年份：2010

卷号：160

期号：3

起止页码：812

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20101112767996);WOS:【SCI-EXPANDED(收录号:WOS:000273845900014)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 50744016), the grant from the Ministry of Science and Technology of China (2007CB707801), and Shanghai Municipal Science and Technology Commission (071607014).

语种：英文

外文关键词：Biosurfactant; Lipopeptide; Structure; Brevibacillus brevis; Surfactin

摘要：Brevibacillus brevis HOB1 was isolated from the formation water of an oil field and found to produce lipopeptides. The separation of lipopeptides was successfully achieved by reversed-phase high-performance liquid chromatography (HPLC) leading to nine separated peaks. The chemical structures of these lipopeptides were studied by means of electrospray ionization mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC/MS), HPLC and electrospray ionization tandem mass spectrometry (ESI-MS/MS). As the results, all the lipopeptides had peptide parts with the same amino acid composition of Asp, Glu, Val, and Leu in the molar ratio 1:1:1:4, while the lipid part was composed of C-13-C-15 beta-hydroxy fatty acids. As the sequence of fraction 1 was determined to be N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C, the same as surfactin, they were proposed to be surfactin isoforms. Fraction 4 (C-15 surfactin) exhibited a good surface activity of 26.8 mN/m with CMC of 9 x 10(-6) M. Surfactin is a powerful biosurfactant possessing biological activities. As far as we know, Br. brevis is a new surfactin-producing species.