文献类型：期刊文献

英文题名：Chromosome-level genome provides novel insights into the starch metabolism regulation and evolutionary history of Tetraselmis helgolandica

作者：Zhou, Zuodong[1];Jiawei, E.[2];Shi, Qianwen[1];Zhang, Wenjun[2];Sun, Liyun[1,2];Fan, Jianhua[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, State Key Lab Incubat Base Green Proc Chem Engn, Shihezi 832003, Peoples R China

年份：2026

卷号：79

起止页码：251

外文期刊名：JOURNAL OF ADVANCED RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001645844700001)】；

基金：This work was sponsored by National Key Research and Development Project of China 2020YFA0907304, "Innovation Yongjiang 2035" Key R&D Programme International Sci-tech Cooperation Projects 2024H002, Natural Science Foundation of Shandong Province ZR2019ZD17, Natural Science Foundation of Shanghai 23ZR1415100 and 24ZR1490800, Funding Project of the State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.

语种：英文

外文关键词：Genome assembly; Comparative genomics; Metabolism; Circadian rhythm; Starch synthesis; Green algae

摘要：Introduction: Tetraselmis helgolandica is a marine microalga belonging to the Chlorophyta phylum. It is widely distributed in the coastal waters of Asia and is commonly used as aquatic feed. T. helgolandica is characterized by its large size, preference for starch accumulation, low temperature tolerance, presence of flagella, and strong motility. However, research on T. helgolandica is limited, and its genome data remains unavailable. Objective: We generated a high-quality, chromosome-scale genome of T. helgolandica. Through comparative genomics, we uncovered the genome characteristics and evolutionary history of T. helgolandica. Additionally, by integrating transcriptome data, we elucidated how the light-dark rhythm enhances the high starch production. Methods: We utilized long-read sequencing data and high-throughput chromosome conformation capture data from the Oxford Nanopore platform to construct a high-quality genome of T. helgolandica. Genome annotation was performed using multiple databases, and comparative genomic analysis was conducted with nine species, including Arabidopsis thaliana, to reveal the evolutionary history. Finally, we combined transcriptome data to elucidate the molecular mechanisms underlying the high starch yield. Results: Circadian rhythm chromosome-scale genome 1,017 million years ago. Thi the green lineage. It may developed Golgi apparatu significantly promote starch accumulation and increase amylose content. The revealed it shares a common ancestor with other green algae approximately s relatively ancient divergence underscores its evolutionary distinction within possess a more complex protein modification mechanism and a more fully s. Circadian rhythm broadly up-regulates key enzymes involved in starch synthesis, including GBSS and Starch Synthase, while down-regulating SS Illa. This regulation enhances starch accumulation and increases the amylose content.
Conclusion: This study provided a high-quality genome of T. helgolandica and revealed the potential mechanism by which the circadian rhythm promotes starch accumulation and increases the amylose ratio. The genome of T. helgolandica will serve as an important resource for evolutionary research and transgenic platform development.
2025 The Author(s). Published by Elsevier B.V. on behalf of Cairo University. This is an open access
article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)