文献类型：期刊文献

英文题名：Improvement of Ethanol Productivity and Energy Efficiency by Degradation of Inhibitors Using Recombinant Zymomonas mobilis (pHW20a-fdh)

作者：Dong, Hong-Wei[1,2];Fan, Li-Qiang[1];Luo, Zichen[1];Zhong, Jian-Jiang[3];Ryu, Dewey D. Y.[4];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Res Inst Chem Ind, Shanghai, Peoples R China;[3]Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China;[4]Univ Calif Davis, Biochem Engn Program, Davis, CA 95616 USA

年份：2013

卷号：110

期号：9

起止页码：2395

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20133116574167);WOS:【SCI-EXPANDED(收录号:WOS:000329277500013)】；

基金：Contract grant sponsor: National Basic Research Program of China Contract grant number: 2011CB707406 Contract grant sponsor: National High-Tech Program of China Contract grant number: 2012AA022301 Contract grant sponsor: Fundamental Research Funds for the Central Universities of China Contract grant number: WF0913005; 1114054; 1214025 Contract grant sponsor: Shanghai Leading Academic Discipline Project Contract grant number: B505

语种：英文

外文关键词：recombinant Z; mobilis ZM4 (pHW20a-fdh); formate dehydrogenase (fdh); NADH regeneration; growth inhibitor; inhibitor degradation; corn stover hydrolysate (CSH)

摘要：Toxic compounds, such as formic acid, furfural, and hydroxymethylfurfural (HMF) generated during pretreatment of corn stover (CS) at high temperature and low pH, inhibit growth of Zymomonas mobilis and lower the conversion efficiency of CS to biofuel and other products. The inhibition of toxic compounds is considered as one of the major technical barriers in the lignocellulose bioconversion. In order to detoxify and/or degrade these toxic compounds by the model ethanologenic strain Z. mobilis itself in situ the fermentation medium, we constructed a recombinant Z. mobilis ZM4 (pHW20a-fdh) strain that is capable of degrading toxic inhibitor, formate. This is accomplished by cloning heterologous formate dehydrogenase gene (fdh) from Saccharomyces cerevisiae and by coupling this reaction of NADH regeneration reaction system with furfural and HMF degradation in the recombinant Z. mobilis strain. The NADH regeneration reaction also improved both the energy efficiency and cell physiological activity of the recombinant organism, which were definitely confirmed by the improved cell growth, ethanol yield, and ethanol productivity during fermentation with CS hydrolysate. Biotechnol. Bioeng. 2013; 110:2395-2404. (c) 2013 Wiley Periodicals, Inc.