文献类型：期刊文献

英文题名：Purification of lignans from Fructus Arctii using off-line two-dimensional supercritical fluid chromatography/reversed-phase liquid chromatography

作者：Yang, Bichao[1];Xin, Huaxia[1];Wang, Feier[1];Cai, Jianfeng[1];Liu, Yanfang[2];Fu, Qing[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Minist Educ, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

年份：2017

卷号：40

期号：16

起止页码：3231

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20172803942172);WOS:【SCI-EXPANDED(收录号:WOS:000409434100006)】；

基金：This work was supported by National Natural Science Funds (21305138) and Fundamental Research Funds for the Central Universities (222201717016).

语种：英文

外文关键词：lignans; reversed-phase liquid chromatography; supercritical fluid chromatography; traditional Chinese medicine; two-dimensional chromatography

摘要：As a common traditional Chinese medicine, Fructus Arctii has important clinical medical values. Its main components are lignans, which are difficult to separate and analyze because of the complex composition, similar chemical structures, and close properties. In this study, an off-line two-dimensional supercritical fluid chromatography/reversed-phase liquid chromatography method, as well as an effective sample pretreatment method based on hydrophilic interaction chromatography material, was developed to enrich the minor lignan fractions and obtain high-purity compounds. In total, 12 high-purity compounds were isolated from Fructus Arctii. Their structures were identified by using high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy, which showed that all were lignans and that most of them were isomers. The results demonstrated the effective off-line two-dimensional supercritical fluid chromatography/reversed-phase liquid chromatography method for the purification of lignans from Fructus Arctii. The separation protocol established here will be beneficial for the separation of complex samples from other kinds of natural products.